分析化学2026，Vol.54Issue(4)：511-520,10.DOI:10.19756/j.issn.0253-3820.261006
基于纳米针阵列的活细胞生物标志物检测与分析研究进展
Advances in Nanoneedle-based Live-Cell Biomarker Sensing and Analysis
摘要
Abstract
Traditional live-cell molecular detection techniques are often impeded by the challenges such as molecular perturbation and probe-induced toxicity.Leveraging their superior biocompatibility,nanomaterials offer a novel paradigm for live-cell interrogation.This review focused on nanoneedle arrays,systematically outlining their principles,applications,and prospects for biomarker analysis.Material compositions,morphological features,and surface functionalization strategies of these arrays were elucidated in detail,alongside the mechanisms governing molecular capture and signal transduction.Specifically,the capability of nanoneedle arrays for in situ detection of RNA,proteins,and metabolites were critically evaluated.Furthermore,the technology's advantages in spatiotemporal analysis were highlighted and the hurdles facing clinical translation were dissected.Ultimately,this paper aimed to establish a robust methodological foundation for deciphering single-cell heterogeneity,exploring disease mechanisms,and elucidating drug-response profiles.关键词
纳米针/活细胞分析/原位检测/单细胞检测/评述Key words
Nanoneedles/Live-cell analysis/In situ detection/Single-cell detection/Review引用本文复制引用
刘艳多,杨德元,孔玉彤,罗立强,王子迅,裴仁军..基于纳米针阵列的活细胞生物标志物检测与分析研究进展[J].分析化学,2026,54(4):511-520,10.基金项目
国家自然科学基金项目(Nos.32471464,22577150)、江苏省自然科学基金项目(Nos.BK20243004,BK20241822,BK2023204)和苏州市自然科学基金项目(Nos.ZXL2023203,SSD2025007)资助. Supported by the National Natural Science Foundation of China(Nos.32471464,22577150),the Natural Science Foundation of Jiangsu Province(Nos.BK20243004,BK20241822,BK2023204)and the Natural Science Foundation of Suzhou(Nos.ZXL2023203,SSD2025007). （Nos.32471464,22577150）
