分析化学2026，Vol.54Issue(4)：743-750,8.DOI:10.19756/j.issn.0253-3820.261017
鲁米诺/二氧化硫脲流动注射化学发光灵敏检测亚硝酸钠
Luminol-Thiourea Dioxide Flow Injection Chemiluminescence for Sensitive Detection of Sodium Nitrite
摘要
Abstract
Sodium nitrite(NaNO2)is widely used as a food additive.Long-term excessive intake of NaNO2 may induce diseases such as gastric cancer,while acute overconsumption can lead to methemoglobinemia which shows severe acute poisoning symptoms.Therefore,rapid and accurate detection of NaNO2 is crucial for preventing acute poisoning as well as monitoring long-term excessive intake-induced diseases.Chemiluminescence(CL)is a method which has advantages of compact instrumentation,simplicity of detection,and high sensitivity.Combining with the benefits of using thiourea dioxide as a luminol co-reagent to enhance the stability and selectivity of the CL system,a luminol/thiourea dioxide flow injection CL system was employed for quantitative analysis of NaNO2.The significant reduction in luminescence upon addition of NaNO2 demonstrated the feasibility of this analytical method.Optimal detection conditions were further established by optimizing solution pH value and flow rate of peristaltic pump.The system was proved suitable for quantifying NaNO2 from 10 nmol/L to 5 mmol/L,with a detection limit(S/N=3)of 3.5 nmol/L.The linear correlation coefficient(r)was 0.9971.A relative standard deviation of 4.36%was obtained for nine consecutive injections of 10 μmol/L NaNO2,indicating a good reproducibility.Furthermore,the addition of six representative interferents showed no significant effect on CL intensity,indicating a good selectivity.关键词
亚硝酸钠/流动注射化学发光/鲁米诺/二氧化硫脲Key words
Sodium nitrite/Flow injection chemiluminescence/Luminol/Thiourea dioxide引用本文复制引用
张巍,肖金威,郑渝,徐国宝..鲁米诺/二氧化硫脲流动注射化学发光灵敏检测亚硝酸钠[J].分析化学,2026,54(4):743-750,8.基金项目
国家自然科学基金项目(No.22174136)和吉林省自然科学基金-国家重点实验室(学科类)重大专项项目(No.SKL202302031)资助. Supported by the National Natural Science Foundation of China(No.22174136)and the Natural Science Foundation of Jilin Province(No.SKL202302031). （No.22174136）
