亚热带农业研究2026，Vol.22Issue(1)：10-20,11.DOI:10.13321/j.cnki.subtrop.agric.res.2026.01.002
甘蔗耐寒相关基因ScERF118的克隆与表达分析
Cloning and expression analysis of the cold tolerance-associated gene ScERF118 in sugarcane
摘要
Abstract
[Purpose]This study cloned and characterized an ERF transcription factor gene associated with cold tolerance in sugar-cane,aiming to provide a candidate target for genetic improvement of sugarcane for cold tolerance.[Method]On the basis of a sug-arcane cold tolerance-associated ERF transcript previously identified by our population-based linkage mapping,the cDNA sequence of ScERF118 was obtained from the sugarcane cultivar YT93-159 via homology-based cloning.The physicochemical properties,phy-logenetic relationship,conserved domains,and promoter cis-acting elements(CREs)of the protein encoded by ScERF118 were ana-lyzed by bioinformatics tools.Expression profiles of the gene under various stress conditions were examined by transcriptomic data and RT-qPCR.Subcellular localization of ScERF118 was determined via a protoplast transient expression system.[Result]The cD-NA sequence of ScERF118 was 1 529 bp in length and contained an open reading frame of 1 053 bp,encoding 350 amino acid resi-dues.The predicted protein was an unstable,acidic,and hydrophilic protein without a signal peptide or transmembrane domain.Subcellular localization results showed that ScERF118 was localized to the nucleus.Phylogenetic analysis revealed that ScERF118 belonged to the B-1 subgroup of the ERF subfamily.The 14th alanine(A)and 19th asparagine(N)residues within the AP2 do-main were conserved across multiple species.The promoter region of the ScERF118 homologous gene contained abundant CREs re-sponsive toabscisic acid,ethylene,and methyl jasmonate,as well as low-temperature and drought stress-responsive elements such as DRE and LTR.Transcriptomic data showed that ScERF118 expression was very low across various sugarcane tissue samples but in-duced by low temperature,drought,and smut pathogen stresses.RT-qPCR validation demonstrated that the expression of ScERF118 was generally upregulated under low temperature stress,with a stronger response observed in cold-tolerant varieties than in cold-sen-sitive ones.[Conclusion]ScERF118 is a nuclear-localized,non-constitutively expressed transcription factor responsive to low tem-perature and other stress signals,potentially playing a regulatory role in the response to low temperature stress.This study lays a foundation for functional characterization of ScERF118 and provides a candidate target for cold tolerance breeding in sugarcane.关键词
甘蔗/ERF 转录因子/低温胁迫/表达分析/亚细胞定位Key words
sugarcane(Saccharum spp.)/ERF transcription factor/low temperature stress/expression analysis/subcellular local-ization分类
农业科技引用本文复制引用
张玥,黄佳妮,曾恒敬,何丝丝,阙友雄,苏亚春..甘蔗耐寒相关基因ScERF118的克隆与表达分析[J].亚热带农业研究,2026,22(1):10-20,11.基金项目
国家重点研发项目"特色经济作物耐逆调控基因挖掘与利用"(2023YFD1200702) （2023YFD1200702）
福建农林大学校级大学生创新训练项目"甘蔗AP2转录因子的抗逆功能分析"(FAFUXMPC20250429001-00305). （FAFUXMPC20250429001-00305）
