亚热带农业研究2026，Vol.22Issue(1)：39-46,8.DOI:10.13321/j.cnki.subtrop.agric.res.2026.01.005
秋水仙素对天贵卷瓣兰多倍体的诱导效应
Induction effect of colchicine on polyploids of Bulbophyllum tianguii
摘要
Abstract
[Purpose]Colchicine was used to induce polyploidy in Bulbophyllum tianguii,providing reliable techniques and meth-ods for germplasm innovation and genetic breeding.[Method]Different concentrations of colchicine were used as mutagens.Clus-tered buds of in vitro B.tianguii plantlets were treated via the soaking and mixed-culture methods.The mutagenic effects were sys-tematically evaluated through flow cytometry,morphological observation,and root-tip cell chromosome counting.[Result]Numer-ous chimeras were induced through both the soaking and mixed-culture methods.The soaking method yielded more tetraploids.The treatment with the highest induction rate(6.67%)was soaking in 150 mg·L-1 colchicine for 12 h,while it showed a chimera rate of 38.33%and a mortality rate of 36.67%.Successfully induced polyploid plants showed significant differences in DNA fluorescence intensity absorption peaks compared with diploids.In terms of leaf and stomatal morphology,tetraploid plants had darker green leav-es,longer and wider leaves,and larger gurd cells(both in length and width)compared with diploid plants.However,their stomatal density(14 per field of view)was significantly lower than that of diploid plants(24 per field of view).Root-tip cell chromosome counting showed that the chromosome number was 2n=2x≈44 in diploid plants and 2n=4x≈88 in tetraploid plants.[Conclusion]Colchicine can successfully induce polyploidy in B.tianguii.The induction effects are influenced by colchicine concentration and treatment duration.The polyploid plants exhibit superior phenotypic traits compared with diploids.关键词
天贵卷瓣兰/秋水仙素/多倍体诱导/倍性育种Key words
Bulbophyllum tianguii/colchicine/polyploid induction/ploidy breeding分类
农业科技引用本文复制引用
农融融,王雅珂,柴胜丰,李伦壮,仇硕..秋水仙素对天贵卷瓣兰多倍体的诱导效应[J].亚热带农业研究,2026,22(1):39-46,8.基金项目
国家重点研发计划课题"基岩生境植被改造与可持续恢复技术"(2022YFF1300703) （2022YFF1300703）
广西重点研发计划项目"桂北高寒山区'冷背'药用植物资源收集、保存及其药材品质提升技术研究"(桂科AB24010138) （桂科AB24010138）
广西林业科技推广示范项目"雅长特色兰科植物天贵卷瓣兰的种苗繁育及回归引种研究"(桂林科字[2021]第28号). （桂林科字[2021]第28号）
