广东农业科学2026，Vol.53Issue(3)：42-52,11.DOI:10.16768/j.issn.1004-874X.2026.03.004
池塘循环流水养殖不同密度罗非鱼的生长及生理生化指标分析
Analysis of Growth and Physiological and Biochemical Indexes of Tilapia in Recirculating Ponds with Different Stocking Densities
摘要
Abstract
[Objective]This study aimed to investigate the effects of different stocking densities on growth performance and physiological and biochemical indexes of tilapia in an In-Pond Raceway System(IPRS),and to determine the appropriate stocking density for this culture system.[Method]Three density gradients were established in separate raceways of the IPRS:Group A(164 fish/m2),Group B(136 fish/m2),and Group C.A traditional pond aquaculture(TPA)system stocked at 3 fish/m2 served as the control(Group D).The growth performance,serum biochemical parameters,and antioxidant stress levels of tilapia in each group were measured.[Result]After 138 days of culture,growth performance in the IPRS groups showed a clear declining trend with increasing density.Group C performed best,with final body weight(FBW),specific growth rate(SGR),and absolute growth rate(AGR)not significantly different from those of the control(P＞0.05),and superior to those of Groups A and B.Feed utilization rate was markedly improved under IPRS,as evidenced by lower feed conversion ratios(FCR)across all IPRS groups compared to the control.Serum biochemical parameters varied with density:acid phosphatase(ACP)and aspartate aminotransferase(AST)activities,as well as glucose(GLU)content generally increased from C to B and then decreased toward A,whereas the total cholesterol(T-CHO)content showed a decreasing trend.Alkaline phosphatase(AKP)and T-CHO activities were significantly higher in all IPRS groups than in the control(P＜0.05),with Group C maintaining relatively balanced activity levels.Alanine aminotransferase(ALT)and creatinine(CRE)concentrations rose steadily with increasing density and were significantly elevated in all IPRS groups relative to the control(P＜0.05).Antioxidant responses differed between tissues.In brain tissue,superoxide dismutase(SOD)and catalase(CAT)activities tended to decrease with rising density,while glutathione peroxidase(GSH-Px)activity first declined and then increased;SOD,CAT and GSH-Px in Group C was 147.84％,143.72％,and 132.81％higher than in Group A,respectively.In liver tissue,SOD and GSH-Px activities increased with density,whereas CAT activity rose initially and then fell.Malondialdehyde(MDA)contents in both brain and liver were significantly lower in all IPRS groups than in the control(P＜0.05),indicating that the IPRS environment did not induce oxidative damage.[Conclusion]Under the conditions of this experiment,a stocking density of 118 fish/m2 is recommended for tilapia reared in IPRS.At this density,although serum ALT and AST levels were higher than in traditional pond culture,they remained within physiologically tolerable ranges.Fish exhibited good adaptation to the recirculating raceway environment,maintained strong growth performance,and achieved a favorable balance between production efficiency and health status.关键词
罗非鱼/池塘循环流水养殖模式/养殖密度/生长性能/生理生化指标/生理应激水平Key words
tilapia/in-pond raceway system/stocking density/growth performance/physiological and biochemical indexes/physiological stress levels分类
农业科技引用本文复制引用
马之瑞,陈丽婷,何智昌,陈旭,严欣,郭忠宝,肖俊,李丹,罗永巨..池塘循环流水养殖不同密度罗非鱼的生长及生理生化指标分析[J].广东农业科学,2026,53(3):42-52,11.基金项目
国家现代农业产业技术体系建设专项(CARS-46) （CARS-46）
广西水产遗传育种与健康养殖重点实验室自主研究课题(2024-A-01-01) （2024-A-01-01）
广西壮族自治区水产科学研究院重点实验室开放课题(GXKEYLA-2023-01-5) （GXKEYLA-2023-01-5）
