中国妇幼健康研究2026，Vol.37Issue(5)：64-69,6.DOI:10.3969/j.issn.1673-5293.2026.05.009
HPV诱导的miR-21-5p通过PI3K/AKT途径促进宫颈癌细胞的上皮间质转化和肿瘤进展
HPV-induced miR-21-5p promotes epithelial-mesenchymal transition and tumor progression in cervical cancer cells via the PI3K/AKT pathway
摘要
Abstract
Objective To investigate the effects of HPV-induced miR-21-5p on epithelial-mesenchymal transition(EMT)and tumour progression in cervical carcinoma cells.Methods HPV(+)cervical cancer cells were divided into the mimic-NC group,miR-21-5p-mimic group,inhibitor-NC group,and miR-21-5p-inhibitor group.Real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to analyze the expression of miR-21-5p in HPV(+)cervical cancer cells,and Transwell assay was detected cell migration and invasion.The expression of E-cadherin,N-cadherin,Vimentin,phosphorylated phosphatidylinositol 3-kinase(p-PI3K),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT)proteins and β-actin were detected by Western blot.Results Compared with HPV(-)C33A cells,the expression of miR-21-5p was significantly increased in HPV16(+)SiHa and HPV18(+)HeLa cells(F=51.35,P<0.01).Transwell assay results demonstrated that,compared with the mimic-NC group,the miR-21-5p-mimic group exhibited significantly increased cell migration and invasion(t=6.73 and 5.64,respectively P<0.01);whereas compared with the inhibitor-NC group,cell migration and invasion were markedly reduced in the miR-21-5p-inhibitor group(t=17.32 and 15.46,respectively P<0.01).Western blot analysis revealed that,compared with the mimic-NC group,miR-21-5p-mimic-treated cells exhibited reduced E-cadherin expression alongside increased N-cadherin and Vimentin expression.Conversely,compared with the inhibitor-NC group,miR-21-5p-inhibitor group cells demonstrated elevated E-cadherin expression alongside decreased N-cadherin and vimentin expression.Compared with the mimic-NC group,p-PI3K and p-AKT levels were significantly elevated in miR-21-5p-mimic group cells.Conversely,compared with the inhibitor-NC group,p-PI3K and p-AKT levels were markedly reduced in miR-21-5p-inhibitor group cells.Conclusion HPV-induced miR-21-5p may promote migration,invasion and epithelial-mesenchymal transition in cervical cancer cells by activating the PI3K/AKT pathway.关键词
HPV/miR-21-5p/PI3K/AKT途径/宫颈癌/上皮间质转化Key words
HPV/miR-21-5p/PI3K/AKT pathway/cervical cancer/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
徐丽娜,崔梦瑶,袁向珍..HPV诱导的miR-21-5p通过PI3K/AKT途径促进宫颈癌细胞的上皮间质转化和肿瘤进展[J].中国妇幼健康研究,2026,37(5):64-69,6.基金项目
内蒙古医科大学青年创新基金项目(YKD2018QNCX072) （YKD2018QNCX072）
