医学分子生物学杂志2026，Vol.23Issue(3)：258-265,8.DOI:10.3870/j.issn.1672-8009.2026.03.004
活化血小板对内皮细胞凋亡损伤的作用研究
Effect of Platelet Activation on Apoptosis and Damage of Endothelial Cells
摘要
Abstract
Objective To investigate the effect of platelet activation on the apoptosis of endo-thelial cells.Methods Platelets were prepared from venous blood,obtained from healthy sub-jects.Resting platelet suspension,activating platelet suspension,and endothelial cells were cul-tured in vitro to establish the co-culture model of platelets and endothelial cells.The aggregation,adhesion and release process of platelets after activation and the apoptosis process of endothelial cells were observed by live-cell imaging technique.The endothelial cell survival activity and mitochondrial membrane potential were then detected by kits.Results Activated platelets exhibited significant ag-gregation,adhesion,and release responses.Compared to resting platelets,activated platelets ag-gregated,migrated toward endothelial cells,and adhered to them.Live-cell imaging revealed that activated platelets induced nuclear pyknosis,condensation,marginalization,reduced nuclear vol-ume,fragmentation,and the formation of apoptotic bodies in endothelial cells.Compared to the resting platelet group,the activated platelet group showed reduced endothelial cell viability and mi-tochondrial membrane potential.Conclusion Platelet activation induces apoptotic injury in endothe-lial cells.关键词
血小板/内皮细胞/凋亡/活细胞成像系统Key words
platelets/endothelial cells/apoptosis/live-cell imaging system分类
医药卫生引用本文复制引用
刘红利,李坤,岳雯,位庚,梁俊清,田金悦..活化血小板对内皮细胞凋亡损伤的作用研究[J].医学分子生物学杂志,2026,23(3):258-265,8.基金项目
国家重点研发计划"中医药现代化研究"重点专项(No.2017YFC1700500),河北省中医药管理局(No.2022488) This work was supported by grants from the National Key R&D Program's Key Project on"Research on the Modernization of Traditional Chinese Medi-cine"(No.2017YFC1700500),Hebei Provincial Administration of Traditional Chinese Medicine(No.2022488). （No.2017YFC1700500）
