医学分子生物学杂志2026，Vol.23Issue(3)：275-284,10.DOI:10.3870/j.issn.1672-8009.2026.03.006
长链非编码RNA与mRNA调控网络参与急性冠脉综合征发病进程并影响关键信号通路
Long Non-Coding RNA-mRNA Regulatory Networks Involved in the Pathogenesis of Acute Coronary Syndrome and Their Impact on Key Signaling Pathways
摘要
Abstract
Objective To identify key molecular biomarkers of acute coronary syndrome(ACS)through transcriptome sequencing and construction of mRNA-lncRNA co-expression net-works.Methods An ACS mouse model was established,and the modeling outcomes and cardiac pathological changes were comprehensively evaluated using physiological and pathological as-says.RNA sequencing(RNA-seq)was performed to obtain gene expression profiles across different samples.Bioinformatics analysis was then applied to explore gene expression changes and functional signaling pathways associated with ACS.Results After ACS modeling,experimental results such as HE staining,TTC staining,and Doppler ultrasound all indicated that ACS led to histopathological changes and dysfunction in mouse cardiac tissue.RNA-seq results revealed 2 557 DEGs that were co-upregulated in both ACS-M and ACS-S,and 1 930 DEGs that were co-downregulated,including mRNAs(DEmRNAs)and lncRNAs(DElncRNAs).Further GO functional enrichment analysis showed that these DElncRNAs regulated the differential expression of target genes related to mito-chondrial function,fatty acid metabolism,immune/inflammatory response,cell adhesion,apopto-sis,fibrosis,and other functions.Additionally,six expression patterns closely associated with ACS were identified.Among them,lncRNAs Dnm3os and Gm13054 were persistently highly expressed in ACS and their high expression characteristics were validated by RT-qPCR.Their downstream targets were closely related to mitochondrial function and tissue fibrosis.Gm13054 also exhibited potential regulatory functions for Nppa expression.Conclusion This study identified a large number of DEm-RNAs and DElncRNAs through RNA-seq,which have potential functions tightly associated with ACS.The construction of a co-expression network between mRNAs and lncRNAs provides potential therapeutic targets and a foundational basis for further clinical research on the diagnosis and treat-ment of ACS.关键词
急性冠脉综合征/转录组测序/长链非编码RNA/差异表达基因Key words
acute coronary syndrome/transcriptome sequencing/long non-coding RNA/dif-ferentially expressed genes分类
医药卫生引用本文复制引用
权有武,陈元亚,吴政杰,刘铠雄,叶远征..长链非编码RNA与mRNA调控网络参与急性冠脉综合征发病进程并影响关键信号通路[J].医学分子生物学杂志,2026,23(3):275-284,10.基金项目
省部共建中亚高发病成因与防治国家重点实验室-巴楚县人民医院联合项目开放课题(No.SKL-HIDCA-2022-BC3) This work was supported by a grant from the Open Project of the Joint Program of State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia(jointly established by the Ministry and the Province)-Bachu County People's Hospital(No.SKL-HIDCA-2022-BC3). （No.SKL-HIDCA-2022-BC3）
