国际神经病学神经外科学杂志2026，Vol.53Issue(2)：26-33,8.DOI:10.16636/j.cnki.jinn.1673-2642.2026.02.004
沉默信息调节因子1与Toll样受体4信号通路在1-甲基-4-苯基-吡啶离子帕金森病细胞模型炎症反应中的作用
Role of the silent information regulator 1-Toll-like receptor 4 signaling pathway in inflammatory response in a cell model of Parkinson disease induced by 1-methyl-4-phenyl pyridine ion
摘要
Abstract
Objective To investigate the role of the silent information regulator 1(Sirt1)-Toll-like receptor 4(TLR4)signaling pathway in inflammatory response in a cell model of Parkinson disease(PD)induced by 1-methyl-4-phenyl pyridine ion(MPP+).Methods SH-SY5Y cells were treated with MPP+to establish a cell model of PD,and resveratrol(RV)and EX527 were used to modulate the expression of Sirt1 in the cell model.SH-SY5Y cells were divided into control group,model group(MPP+group),activator group(RV+MPP+group),and inhibitor group(EX527+MPP+group),and the cells were cultured in a high-glucose medium.CCK-8 assay was used to assess the cytotoxicity of MPP+,RV,and EX527,and finally MPP+(0.75 mmol/L),RV(20 μmol/L),and EX527(80 μmol/L)were selected for 48 hours of intervention.Western blotting was used to measure the expression levels of tyrosine hydroxylase(TH),Sirt1,and the inflammation-related protein TLR4;ELISA was used to measure the levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in cell supernatant;immunofluorescence assay was used to measure the expression of TH,Sirt1,TLR4,and related inflammatory factors in cells.Results Compared with the control group,the MPP+group had significant reductions in the expression levels of TH and Sirt1 and a significant increase in the expression level of TLR4(P<0.05);compared with the MPP+group,the RV+MPP+group had significant increases in the expression levels of TH and Sirt1 and a significant reduction in the expression level of TLR4(P<0.05);compared with the control group and the RV+MPP+group,the EX527+MPP+group had significant reductions in the expression levels of TH and Sirt1 and a significant increase in the expression level of TLR4(P<0.05).ELISA showed that compared with the control group and the RV+MPP+group,the MPP+group and the EX527+MPP+group had significant increases in the levels of IL-1β,IL-6,and TNF-α(P<0.05),and compared with the MPP+group,the EX527+MPP+group had significant increases in the levels of IL-1β,IL-6,and TNF-α(P<0.05).Immunofluorescence assay showed that compared with the control group,the MPP+group had significant reductions in the expression levels of TH and Sirt1 and significant increases in the expression levels of TLR4 and the inflammatory factors IL-1β and TNF-α(P<0.01);compared with the MPP+group,the RV+MPP+group had significant increases in the expression levels of TH and Sirt1 and significant reductions in the expression levels of TLR4,IL-1β,and TNF-α(P<0.01);compared with the MPP+group,the EX527+MPP+group had significant reductions in the expression levels of TH and Sirt1 and significant increases in the expression levels of TLR4 and the inflammatory factors IL-1β and TNF-α(P<0.01).Conclusions The Sirt1-TLR4 signaling pathway is significantly associated with the process of neuroinflammation in PD and may play a role in the development and progression of the disease.关键词
帕金森病/沉默信息调节因子1/Toll样受体4/炎症反应/1-甲基-4-苯基-吡啶离子Key words
Parkinson disease/silent information regulator 1/Toll-like receptor 4/inflammatory response/1-methyl-4-phenyl pyridine ion分类
医药卫生引用本文复制引用
郑婷华,邢瑶平,陈敏,郭彦杰,邢红霞..沉默信息调节因子1与Toll样受体4信号通路在1-甲基-4-苯基-吡啶离子帕金森病细胞模型炎症反应中的作用[J].国际神经病学神经外科学杂志,2026,53(2):26-33,8.基金项目
2021年度河南省医学科技攻关计划联合共建项目(LHGJ20210548) （LHGJ20210548）
2025年度河南省政府决策研究招标课题"河南康复作业治疗学人才培养的路径探索与实践研究"(2025JC093) （2025JC093）
2024年度河南省医学科技攻关计划联合共建项目(LHGJ20240510) （LHGJ20240510）
河南省2025年科技发展计划河南省科技攻关项目(252102310279). （252102310279）
