南方农业学报2026，Vol.57Issue(3)：607-620,14.DOI:10.3969/j.issn.2095-1191.2026.03.001
荔枝蒂蛀虫2个气味结合蛋白基因克隆及其与性信息素的分子对接
Cloning of two odorant binding protein genes in Conopomorpha sinensis Bradley and their molecular docking with sex pheromone components
摘要
Abstract
[Objective]This study clarified the function of odorant binding proteins(OBPs)of Conopomorpha sinen-sis Bradley in the recognition of sex pheromones,which could provide theoretical basis for elucidating the chemical com-munication mechanism underlying sex pheromone recognition in Conopomorpha sinensis Bradley.[Method]Based on transcriptome data from the antennae of female and male Conopomorpha sinensis Bradley,the CsOBPs proteins related to the recognition of sex pheromones were obtained,rapid amplification of cDNA end(RACE)technique was adopted to clone the full-length of CsOBPs gene and bioinformatics analysis was also conducted.The expression patterns of CsOBPs gene in different tissues(antennae,head,thorax,abdomen,leg,wing)and time points(00:00,04:00,08:00,12:00,16:00,20:00,24:00)of the 3-day-old adult of Conopomorpha sinensis Bradley were detected by real-time fluorescence quantitative PCR.The tertiary structure model of the CsOBPs protein was constructed using AlphaFold 2,and its binding ability with sex pheromones was analyzed through molecular docking.[Result]Two sex pheromone recognition-related OBPs genes sequences,CsOBP1 and CsOBP2,were successfully cloned.The full-length gene sequences were obtained through amplification.CsOBP1 gene was 859 bp in length,encoding 121 amino acids residues with a molecular weight of 14.27 kD and theoretical isoelectric point of 5.91.CsOBP2 gene was 824 bp in length,encoding 160 amino acids residues with a molecular weight of 18.24 kD and theoretical isoelectric point of 5.27.Real-time fluorescence quantitative PCR analysis revealed that,both genes were expressed in various tissues of Conopomorpha sinensis Bradley,with the highest expression level in male antennae.Additionally,the expression of the two genes peaked at night,which was consistent with the mating behavior rhythm of this pest.The results of homology modeling showed that CsOBP1 and CsOBP2 pro-teins possessed the typical characteristics of OBPs,containing three pairs of disulfide bonds formed by six conserved cys-teine residues.The results of molecular docking indicated that CsOBP1 and CsOBP2 proteins had strong binding ability for six sex pheromone components with binding energies ranging from-5.3 to-9.1 kcal/mol.Molecular dynamics trajec-tory simulation results indicated that CsOBP1 and CsOBP2 proteins formed stable complexes with sex pheromone compo-nents primarily through van der Waals interaction energy and nonpolar solvation energy.[Conclusion]CsOBP1 and CsOBP2 genes are associated with sex pheromone recognition,and CsOBP1 and CsOBP2 can act as sex pheromone binding proteins involved in regulating the process of sex pheromone recognition in Conopomorpha sinensis Bradley.关键词
荔枝蒂蛀虫/气味结合蛋白/实时荧光定量PCR/分子对接Key words
Conopomorpha sinensis Bradley/odorant binding proteins/real-time fluorescence quantitative PCR/molecular docking分类
农业科技引用本文复制引用
常虹,王思威,刘艳萍,王潇楠,郭江龙,高燕..荔枝蒂蛀虫2个气味结合蛋白基因克隆及其与性信息素的分子对接[J].南方农业学报,2026,57(3):607-620,14.基金项目
广东省基础与应用基础研究基金项目(2022A1515110473) （2022A1515110473）
广州市科技计划项目(2023A04J0805) （2023A04J0805）
国家荔枝龙眼产业技术体系专项(CARS-32) Guangdong Basic and Applied Fundamental Research Foundation(2022A1515110473) （CARS-32）
Guang-zhou Science and Technology Plant Project(2023A04J0805) （2023A04J0805）
China Agriculture Research System-Litchi and Longan(CARS-32) （CARS-32）
