广西植物2026，Vol.46Issue(4)：586-600,15.DOI:10.11931/guihaia.gxzw202512031
贵州九种猕猴桃属植物叶绿体基因组特征及比较分析
Chloroplast genome characteristics and comparative analysis of nine Actinidia species in Guizhou
摘要
Abstract
To elucidate the chloroplast genomic characteristics and phylogenetic relationships within the genus Actinidia,nine species distributed in Guizhou Province,including A.polygama,A.rubricaulis var.coriacea,and A.callosa var.henryi,were selected in this study.Based on next-generation sequencing data,we performed chloroplast genome assembly,annotation,and comparative genomic analysis to systematically investigate their genomic characteristics and phylogenetic relationships.The results were as follows:(1)The chloroplast genomes of all nine Actinidia species were double-stranded circular molecules with a typical quadripartite structure.Their full lengths ranged from 155 660 to 156 770 bp,and the overall GC content varied from 37.21％to 37.33％,indicating high similarity in genome size.(2)A total of 130 genes were annotated in most species,including 83 protein-coding genes,39 tRNA genes,and 8 rRNA genes,except that 129 genes were annotated in A.fulvicoma and A.fortunatii.(3)Codon usage bias was similar among species,with a preference for A/U at the third codon position.A total of 491 simple sequence repeat(SSR)loci were identified,encompassing six repeat types ranging from mononucleotide to hexanucleotide.(4)Comparative genomic analysis revealed that sequence variation was higher in the large single copy(LSC)and small single copy(SSC)regions than in the inverted repeat(IR)region,and that non-coding regions exhibited more pronounced variation than coding regions.Nine divergent gene fragments were identified,including intergenic spacers such as rps16-trnQ-UUG,ndhC-trnV-UAC,and the rbcL-accD region.(5)Phylogenetic analysis resolved the nine species into four clades:A.polygama was phylogenetically distant from the others;A.chinensis and A.chinensis var.deliciosa clustered together;A.callosa var.henryi,A.rubricaulis var.coriacea,A.fortunatii,and A.fulvicoma formed a distinct clade;and A.latifolia showed the closest relationship with A.eriantha.This study provides an important basis at the chloroplast genome level for the identification and conservation of kiwifruit germplasm resources in Guizhou Province,and also accumulates key data and a theoretical basis for the taxonomy,phylogeny and molecular identification of this genus.关键词
猕猴桃属/叶绿体基因组/简单序列重复/序列比对/系统发育Key words
Actinidia/chloroplast genome/simple sequence repeats/sequence alignment/phylogeny分类
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张晟,史斌斌,刘青,仲伟敏,齐勇,唐冬梅,周嘉..贵州九种猕猴桃属植物叶绿体基因组特征及比较分析[J].广西植物,2026,46(4):586-600,15.基金项目
黔科合支撑项目([2023]一般045) （[2023]一般045）
黔科合重大专项([2024]026) （[2024]026）
黔科合平台项目(ZSYS[2025]027) （ZSYS[2025]027）
国家现代农业产业技术体系项目(CARS-26) （CARS-26）
黔科合基础项目(ZK[2023]一般167). （ZK[2023]一般167）
