广西植物2026，Vol.46Issue(4)：615-629,15.DOI:10.11931/guihaia.gxzw202512020
对萼猕猴桃HMGR基因家族的鉴定及其对淹水胁迫的响应
Identification of the HMGR gene family in kiwifruit(Actinidia valvata)and its response to waterlogging stress
摘要
Abstract
3-Hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)is a key enzyme in the terpenoid biosynthetic pathway and plays a vital role in plant growth,development,and responses to abiotic stress.To investigate the characteristics of the AvHMGR gene family and its function under waterlogging stress,a genome-wide identification of the AvHMGR gene family in Actinidia valvata was performed using bioinformatics approaches.Comprehensive analyses were conducted on their chromosomal localization,gene structures,conserved protein domains,and cis-acting elements in promoter regions,etc.The results were as follows:(1)A total of 12 AvHMGR family members were identified in the A.valvata genome and were located across 12 different chromosomes.These AvHMGR genes encoded proteins ranging from 514 to 597 amino acids in length,all of which were predicted to be localized in the endoplasmic reticulum.Based on the phylogenetic analysis,AvHMGR members were grouped into three clusters.(2)Promoter analysis revealed abundant hormone-responsive elements and stress-responsive elements within the AvHMGR promoters.(3)Expression profiling showed that AvHMGR6b and AvHMGR9a/b exhibiting relatively high expression levels at different tissues and fruit developmental stages;AvHMGR5a/b were up-regulated after 12 h of salt stress,suggesting their involvement in short-term salt stress response.(4)Under waterlogging stress,multiple AvHMGR genes including AvHMGR5b,AvHMGR6a/b,AvHMGR9a/b,and AvHMGR21a were significantly up-regulated in both leaves and roots,suggesting that AvHMGRs played a critical role in plant responses to waterlogging stress.The results of this study provide a theoretical foundation for further research on the functional characterization of HMGR in plant responses to waterlogging stress and future breeding effort aimed at enhancing kiwifruit tolerance to waterlogging stress.关键词
3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)/对萼猕猴桃/全基因组鉴定/表达分析/淹水胁迫Key words
3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)/Actinidia valvata/genome-wide indentification/expression analysis/waterlogging stress分类
农业科技引用本文复制引用
张梅娟,常紫锐,李金玲,刘若男,高建有,刘翠霞,李洁维,王发明..对萼猕猴桃HMGR基因家族的鉴定及其对淹水胁迫的响应[J].广西植物,2026,46(4):615-629,15.基金项目
广西科技重大专项(桂科AA23023008) （桂科AA23023008）
国家自然科学基金(32360729) （32360729）
陕西省大学生创新创业训练计划项目(S202511396074) （S202511396074）
商洛学院科研基金(23KYPY07). （23KYPY07）
