广西植物2026，Vol.46Issue(4)：630-643,14.DOI:10.11931/guihaia.gxzw202601005
革叶猕猴桃休眠过程中生理变化与转录组分析
Physiological changes and transcriptome analysis of Actinidia rubricaulis var.coriacea during dormancy
摘要
Abstract
This study selected Actinidia rubricaulis var.coriacea as the research subject,systematically measuring chilling requirements and integrating physiological-biochemical assays with high-throughput transcriptome sequencing to elucidate dynamic changes in bud carbohydrate metabolism,endogenous hormone balance,and antioxidant enzyme systems during the process from the dormancy initiation to the dormancy release.The results were as follows:(1)With chilling accumulation,bud soluble sugar content significantly increased(approximately two fold of initial stage)while starch content markedly decreased(to about half of initial level)by dormancy release,indicating a dynamic metabolic shift from starch degradation to soluble sugar accumulation.(2)Endogenous hormone levels exhibited stage-specific changes,with abscisic acid(ABA)content decreasing from 486.75 μg·L-1 at initial stage to 218.45 μg·L-1 at dormancy release stage,and gibberellin(GA)content increasing from 214.23 pg·mL-1 to 614.75 pg·mL-1,resulting in an ABA/GA ratio decline from 2.27 to 0.36,suggesting a hormonal shift toward germination promotion.(3)Antioxidant enzyme system(SOD,POD,etc.)activities peaked at 192 h of chilling accumulation,indicating enhanced antioxidant capacity as a response to cold stress.(4)Transcriptome analysis identified 12 685 differentially expressed genes(DEGs),with 6 067 genes up-regulated and 6 618 genes down-regulated,enriched in plant hormone signal transduction,starch and sucrose metabolism,and environmental response pathways,revealing a multi-gene coordinated regulatory mechanism.This study,for the first time at the systems level,elucidates the physiological-transcriptomic coordinated regulatory mechanism of chilling requirement accumulation in A.rubricaulis var.coriacea,providing a foundation for addressing climate warming-induced dormancy disorders and related breeding research.关键词
革叶猕猴桃/需冷量/休眠解除/转录组分析/激素调控Key words
Actinidia rubricaulis var.coriacea/chilling requirement/dormancy release/transcriptome analysis/hormone regulation分类
农业科技引用本文复制引用
夏黎明,王发明,叶开玉,蒋桥生,高建有,李洁维,刘翠霞,朱荣香,龚弘娟,齐贝贝..革叶猕猴桃休眠过程中生理变化与转录组分析[J].广西植物,2026,46(4):630-643,14.基金项目
广西科技重大专项(桂科AA23023008) （桂科AA23023008）
国家现代农业产业技术体系"广西落叶果树产业创新团队"项目(nycytxgxcxtd-2023-13-01,nycytxgxcxtd-2023-13-06) （nycytxgxcxtd-2023-13-01,nycytxgxcxtd-2023-13-06）
广西植物研究所基本业务费项目(桂植业24005) （桂植业24005）
广西植物功能物质研究与利用重点实验室项目(ZRJJ2022-2) （ZRJJ2022-2）
国家自然科学基金(32060666). （32060666）
