工业微生物2026，Vol.56Issue(2)：23-25,3.DOI:10.3969/j.issn.1001-6678.2026.02.008
拉曼单细胞技术在耐冷硝化菌筛选中的应用
Application of Raman Single-cell Technology in the Screening of Cold-resistant Nitrifying Bacteria
摘要
Abstract
As a new type of microbial analysis method,Raman single-cell technology has shown unique advantages in the screening and identification of cold-resistant nitrifying bacteria.This technology realizes non-destructive detection of single cells through Raman spectral characteristics,and can achieve accurate separation of target strains in combination with sorting modules.This study finds that Raman spectroscopy can accurately reflect the metabolic characteristics and activity changes of nitrifying bacteria in low-temperature environments,providing a reliable basis for the rapid screening of cold-resistant strains.By establishing a Raman spectral fingerprint library,the rapid identification and classification of cold-resistant nitrifying bacteria can be achieved.This method significantly improves the screening efficiency of cold-resistant nitrifying bacteria and provides technical support for the optimization of low-temperature biological denitrification processes.关键词
拉曼光谱/单细胞技术/耐冷硝化菌/快速筛选/光谱指纹Key words
Raman spectroscopy/single-cell technology/cold-resistant nitrifying bacteria/rapid screening/spectral fingerprint引用本文复制引用
张蕾蕾..拉曼单细胞技术在耐冷硝化菌筛选中的应用[J].工业微生物,2026,56(2):23-25,3.基金项目
吉林省科技厅重点研发-科技资源开发类项目"基于拉曼单细胞分选技术在耐冷型硝化菌快速鉴定中的方法开发"(编号:20250206039ZP). （编号:20250206039ZP）
