海南医科大学学报2026，Vol.32Issue(9)：668-676,9.DOI:10.13210/j.cnki.jhmu.20250225.004
六味地黄丸调控星形胶质细胞A1/A2极化促进髓鞘再生的作用及机制研究
The efficacy and mechanism of Liuwei Dihuang Pill in regulating the A1/A2 polarization of astrocytes and promoting myelin regeneration
摘要
Abstract
Objective:To explore the efficacy and mechanism of Liuwei Dihuang Pills in promoting remyelination in multiple sclerosis.Methods:A total of sixty C57BL/6J mice were randomly divided into the normal control group,the model group,the Fingolimod group and the Liuwei Dihuang Pill low-dose,medium-dose and high-dose groups,with 10 mice in each group.The mice in the model group and the drug group were fed with a diet containing 0.2%dicyclohexanone oxalyl dihydrazone for 5 weeks to induce demyelination.From the 6th week onwards,the normal diet was resumed.Meanwhile,the mice in the treatment group were given intragastric administration of the drug until the end of the 8th week when samples were collected.The luxol fast blue staining observation myelinated area of change;Immunohistochemical determination of the expression of myelin basic protein(MBP),observe the myelin regeneration;Immunofluorescence determination of astrocyte A1/A2 polarization markers,observe the party works for the regulation of A1/A2 polarization;ELISA method to detect the corresponding cytokine IL-1α and TNF-α.Western blot was used to detect the expression of MBP,C3,S100A10,p65 and p-p65 proteins in mice brain tissues.Results:Af-ter gavage administration of Liuwei Dihuang Pill,the myelin sheath LFB staining and MBP expression of mouse brain tissue in-creased(P<0.01).The expressions of key proteins p65 and p-p65 and related cytokines TNF-α and IL-1α in the NF-κB signaling pathway were decreased(P<0.05).AST polarization towards A1 is reduced,and C3 expression is reduced.The polarization of AST in the direction of A2 increased,and the expression of S100A10 increased.Conclusion:Liuwei Dihuang Pill may significant-ly alleviate neuroinflammation,mediate A1/A2 polarization,and promote remyelination in multiple sclerosis by regulating the NF-κB signaling pathway and related cytokines.关键词
六味地黄丸/多发性硬化/髓鞘再生/星形胶质细胞/NF-κB信号通路Key words
Liuwei Dihuang Pill/Multiple sclerosis/Remylination/Astrocytes/NF-κB signaling pathway分类
医药卫生引用本文复制引用
来林,张利,赵佳豪,柳琳,曹秋实,李德顺,孙雅琴..六味地黄丸调控星形胶质细胞A1/A2极化促进髓鞘再生的作用及机制研究[J].海南医科大学学报,2026,32(9):668-676,9.基金项目
This study was supported by the National Natural Science Foundation of China(82305079) （82305079）
2022 Annual"Talents Development Program"Project of Hubei University of Chinese Medicine(2022ZZXQ010) （2022ZZXQ010）
2022 Annual Doctoral Research Startup Funding Project of Hubei University of Chinese Medicine(2022ZZXB004) 国家自然科学基金(82305079) （2022ZZXB004）
湖北中医药大学2022年度"青苗计划"项目(2022ZZXQ010) （2022ZZXQ010）
湖北中医药大学2022年度博士科研启动经费资助项目(2022ZZXB004) （2022ZZXB004）
