海南医科大学学报2026，Vol.32Issue(9)：707-720,14.DOI:10.13210/j.cnki.jhmu.20250227.004
基于网络药理学和实验验证探讨高尤-7味丸改善急性肾损伤的作用机制
Mechanism of Gaoyou-7 Pill in ameliorating acute kidney injury based on network pharmacology and experimental verification
摘要
Abstract
Objective:To explore the renal protective effects and mechanisms of Gaoyou-7 Pill against acute kidney injury(AKI).Methods:Network pharmacology was employed to identify active ingredients and potential targets of Gaoyou-7 Pill,fol-lowed by PPI network analysis.Common targets of Gaoyou-7 Pill and AKI were subjected to GO and KEGG enrichment analyses using R software.A core component-potential target-disease-signaling pathway network was constructed,and molecular docking was performed between core components and key targets.A LPS-induced AKI mouse model was established to preliminarily eval-uate disease pathology and therapeutic efficacy.Renal histopathological changes were observed via H&E staining,and renal func-tion was assessed using enzymatic assay kits.qRT-PCR was utilized to measure mRNA levels of TNF-α,IL-6,and IL-1β,while Western blot was performed to detect protein expression of PI3K,Akt,and p-Akt.Results:A total of 75 active ingredients of Gaoyou-7 Pill were identified,with 54 potential targets and 7 core components(quercetin,T-muurolol,hexadecanoic acid,alpha caryophyllene,linoleic acid,cis cinnamaldehyde,phenethyl acetate)and 6 core targets(AKT1,TNF,IL6,TP53,IL1B,EG-FR)screened.GO and KEGG analysis revealed significant enrichment in transcription factor activity,cell differentiation,and sig-naling pathways including PI3K/Akt,IL-17,TNF,and HIF-1.In vivo experiments demonstrated that Gaoyou-7 Pill protected renal function,reduced the levels of TNF-α,IL-6,and IL-1β,and upregulated the expression of PI3K and p-Akt/Akt.Conclu-sion:Gaoyou-7 Pill ameliorates AKI through multi-component,multi-target,and multi-pathway mechanisms,primarily by modu-lating the PI3K/Akt pathway and suppressing inflammatory cytokine production,thereby preserving renal function.These findings provide a theoretical foundation for the rational clinical application and further development of Mongolian medicine.关键词
高尤-7味丸/急性肾损伤/网络药理学/分子对接/实验验证Key words
Gaoyou-7 Pill/Acute kidney injury/Network pharmacology/Molecular docking/Experimental verification分类
医药卫生引用本文复制引用
杨雨霏,邬国栋,郭叶,薄彧坤,杨丹,安明..基于网络药理学和实验验证探讨高尤-7味丸改善急性肾损伤的作用机制[J].海南医科大学学报,2026,32(9):707-720,14.基金项目
This study was supported by the National Natural Science Foundation of China(82460807) （82460807）
Natural Science Foundation of Inner Mongolia Autonomous Region(2023MS08046) （2023MS08046）
Central Guidance for Local Science and Technology Projects(2022ZY0167) （2022ZY0167）
Standardization Project of Mongolian Medicine in Inner Mongolia Autonomous Region(2023MB031) 国家自然科学基金(82460807) （2023MB031）
内蒙古自治区自然科学基金项目(2023MS08046) （2023MS08046）
中央引导地方科技项目(2022ZY0167) （2022ZY0167）
内蒙古自治区蒙医药标准化项目(2023MB031) （2023MB031）
