环境工程学报2026，Vol.20Issue(4)：1182-1192,11.DOI:10.12030/j.cjee.202508053
生物表面活性剂强化电动-生物阻隔修复场地多环芳烃污染土壤
Biosurfactant-enhanced electro-biological sequestration remediation for PAH-contaminated soil at site
摘要
Abstract
The application of a biosurfactant is a crucial means to enhance the bioavailability of polycyclic aromatic hydrocarbons(PAHs)in soil.To explore the enhancement effect of biosurfactant on in-situ electro-biological sequestration remediation of PAHs-contaminated soil at site,three single biosurfactants(rhamnolipid,alkyl glycoside,and saponin)and their mixture,all at 10 times the critical micelle concentration(CMC)were selected for laboratory simulation experiments.Meanwhile,the effects of rhamnolipid and alkyl glycoside at 2.5,5.0,7.5,and 10 times the CMC on the enhancement were compared.The results showed that:1)The addition of biosurfactants was beneficial to maintaining soil electrical current and moisture content,and promoted PAH removal from contaminated soil.The efficiency of the mixed surfactants was higher than that of single biosurfactants,and this efficiency increased with increasing surfactant concentration.Among them,the treatment with rhamnolipid and alkyl glycoside at 10 times the CMC(Exp IV)demonstrated the best performance,with soil average electrical current and moisture content being 1.31 times and 1.12 times higher than those in treatment without surfactant(CK),and the amount of PAHs removed from the contaminated soil reached 106.02 mg·kg-1.2)The addition of biosurfactants was conductive to the growth and reproduction of bacterial in the contaminated soil and the biological barrier layer.The treatment with rhamnolipid and alkyl glycoside at 10 times the CMC significantly enhanced bacterial growth,with the bacterial count increasing by 6.24 times and 44.8％respectively compared to the initial number.3)Excessive surfactant concentrations could lead to PAH accumulation in the biological barrier layer and clean soil.The addition rhamnolipid and alkyl glycoside at 5 times the CMC could maximize PAH removal while ensuring the effectiveness of the barrier.The results confirm that biosurfactants at appropriate concentrations can enhance the effect of electro-biological sequestration remediation,and this study can provide technical support for the remediation and safe utilization of PAHs-contaminated sites.关键词
污染土壤/多环芳烃(PAHs)/生物表面活性剂/生物可利用性/细菌数量Key words
contaminated soil/polycyclic aromatic hydrocarbons(PAHs)/biosurfactant/bioavailability/bacterial count分类
资源环境引用本文复制引用
韩亚娜,李景铭,李冰霓,佟梦晗,席凯璐,李凤梅..生物表面活性剂强化电动-生物阻隔修复场地多环芳烃污染土壤[J].环境工程学报,2026,20(4):1182-1192,11.基金项目
沈阳市科技计划资助项目(24-213-3-08) （24-213-3-08）
国家自然科学基金面上资助项目(41977140) （41977140）
