黑龙江动物繁殖2026，Vol.34Issue(2)：1-7,7.DOI:10.19848/j.cnki.ISSN1005-2739.2026.04.0001
胚胎移植当日受体母羊血清代谢组特征与妊娠结局的关联研究
Association between serum metabolomic characteristics on the day of embryo transfer and pregnancy outcomes in recipient ewes
摘要
Abstract
This study aimed to preliminarily explore the association between serum metabolic characteristics of recipient ewes on the day of embryo transfer and subsequent pregnancy outcome.Healthy Mongolian sheep were used as the experimental animals.On the day of embryo transfer,jugular venous blood was collected,and recipient ewes were classified into pregnant group(n=7)and non-pregnant group(n=3)according to pregnancy diagnosis results.Non-targeted metabolomics analysis of serum samples was performed using liquid chromatography tandem mass spectrometry(LC-MS/MS)technology,and principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)models were used for data analysis.The results showed that a total of 21 differential metabolites(VIP>1,P<0.05,FC>2or<0.5)were identified,mainly including S-allyl-L-cysteine,L-alanyl-L-alanyl-L-arginine,glycine dehydrocholic acid,and arachidic acid.KEGG pathway enrichment analysis showed that differential metabolites mainly involved pathways such as biosynthesis of unsaturated fatty acids,vitamin B₆ metabolism,purine metabolism,and biosynthesis of cofactors.There is a certain correlation between the serum metabolomic characteristics of recipient ewes on the day of embryo transfer and pregnancy outcomes.Metabolites such as S-allyl-L-cysteine,L-alanyl-L-alanyl-L-arginine,glycine dehydrocholic acid,and arachidic acid suggest that they are candidate biomarkers for evaluating the pregnancy potential of recipient ewes.关键词
胚胎移植/血清/代谢组学/差异代谢物/妊娠结局Key words
embryo transfer/serum/metabolomics/differential metabolites/pregnancy outcome分类
农业科技引用本文复制引用
李基跃,席广银,李可,范利宏,包梅荣,仇春娟,龚璐,乌日嘎,陈大勇,程磊..胚胎移植当日受体母羊血清代谢组特征与妊娠结局的关联研究[J].黑龙江动物繁殖,2026,34(2):1-7,7.基金项目
内蒙古自治区中央引导地方科技发展资金项目(2024ZY0160) （2024ZY0160）
内蒙古自治区科技突围重大项目(2025ZDSF0018) （2025ZDSF0018）
