黑龙江动物繁殖2026，Vol.34Issue(2)：15-20,6.DOI:10.19848/j.cnki.ISSN1005-2739.2026.04.0003
利木赞牛超数排卵及胚胎移植影响因素探究
Factors influencing superovulation and embryo transfer in Limousin cattle
摘要
Abstract
To investigate the factors influencing the effects of superovulation and embryo transfer in Limousin cattle,a total of 8 Limousin donor cows and 26 Simmental recipient cows were used in this study.Donor cows were superovulated with a CIDR+FSH+PG+GnRH protocol,and recipient cows were synchronized using a GnRH+PG regimen.The numbers of follicles and corpora lutea were determined by B-ultrasonography and rectal palpation.Embryo collection and transfer were conducted by non-surgical methods.Data were statistically analyzed using Spearman rank correlation and Fisher's exact test.The results showed that the number of corpora lutea on the day of embryo flushing was significantly and strongly positively correlated with the number of recovered embryos(rS=0.868,P=0.006).The number of pre-superovulation ovarian follicles in donor cows showed a positive but non-significant tendency with embryo yield(rS=0.476,P=0.236).After estrus synchronization,the pregnancy rate of recipient cows with grade A corpora lutea was 61.11%(11/18),and that with grade B corpora lutea was 50.00%(4/8),showing no significant difference(P=0.683).The pregnancy rate for morula transfer was 52.63%(10/19),and 71.43%(5/7)for blastocyst transfer,showing no significant difference(P=0.658).In conclusion,the number of corpora lutea in donor cows can be an indicator of the embryo production potential.Corpus luteum quality and embryo type in recipient cows have no significant effects on pregnancy rate.关键词
利木赞牛/超数排卵/胚胎移植/影响因素/肉牛繁殖Key words
limousin cattle/superovulation/embryo transfer/influencing factors/beef cattle reproduction分类
农业科技引用本文复制引用
胡晓辰,张晨晖,张子敬,王二耀,吕晨晨,朱肖亭,施巧婷,乔智慧,王香南,张楠驰,栾曼茹,刘奥兵..利木赞牛超数排卵及胚胎移植影响因素探究[J].黑龙江动物繁殖,2026,34(2):15-20,6.基金项目
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
神农种业实验室协同创新项目(SN02-2025-02) （SN02-2025-02）
河南省肉牛产业技术体系项目(HARS-22-13-S) （HARS-22-13-S）
河南省科技攻关项目(252102110073、262102111031、262102110036) （252102110073、262102111031、262102110036）
河南省自然科学基金项目(262300420459) （262300420459）
河南省农业科学院自主创新项目(2026ZC78) （2026ZC78）
河南省农业科学院科技创新团队项目"牛羊繁育与生产"(2024TD25) （2024TD25）
