黑龙江动物繁殖2026，Vol.34Issue(2)：21-30,10.DOI:10.19848/j.cnki.ISSN1005-2739.2026.04.0004
牛羊卵母细胞体外成熟技术研究的文献分析
Bibliometric analysis of research on in vitro maturation techniques of cattle and sheep oocytes
摘要
Abstract
To understand the current research progress in in vitro maturation(IVM)technology of bovine and ovine oocytes,this study conducted keyword co-occurrence,density,and trending topic analyses using vosviewer and bibliometrix based on relevant literature published from 2015 to 2025.The results showed that current research on bovine and ovine oocyte IVM commonly focuses on key issues including insufficient follicular microenvironment support,enhanced oxidative stress,mitochondrial dysfunction,increased apoptosis,and vitrification-induced damage.Specifically,bovine studies emphasize microenvironment reconstruction,extracellular vesicle-mediated communication,and molecular quality evaluation,whereas ovine studies concentrate more on cryopreservation vulnerability,antioxidant protection,and IVM quality improvement of oocytes from different sources.In conclusion,the core bottleneck of bovine and ovine oocyte IVM lies in the difficulty of fully reconstructing the in vivo follicular microenvironment in vitro;future optimization should develop more species-specific culture strategies and multidimensional quality evaluation systems based on a comprehensive understanding of shared mechanisms.关键词
牛/羊/卵母细胞/体内外成熟/卵泡微环境/氧化应激Key words
bovine/ovine/oocyte/in vivo and in vitro maturation/follicular microenvironment/oxidative stress分类
农业科技引用本文复制引用
左叶莱,席广银..牛羊卵母细胞体外成熟技术研究的文献分析[J].黑龙江动物繁殖,2026,34(2):21-30,10.基金项目
内蒙古自治区科技创新重大示范专项(2025ZDSF0018) （2025ZDSF0018）
