黑龙江农业科学Issue(4)：16-24,9.DOI:10.11942/j.issn1002-2767.2026.04.0016
低氮胁迫下大豆根系的激素响应及代谢组学分析
Hormonal Response and Metabolomics Analysis of Soybean Roots Under Low Nitrogen Stress
摘要
Abstract
In order to investigate the physiological mechanism and key differential metabolite regulation patterns of different low-nitrogen tolerant soybean genotypes under low nitrogen stress,the low-nitrogen tolerant cultivar Dongnong 59(Res)and the sensitive cultivar Changji Huangdou(Sus)were used as materials.The responses of root architecture,endogenous hormone contents,and metabolites to low nitrogen stress were analyzed under field conditions.The results showed that Dongnong 59 maintained better root growth,chlorophyll content,and root/shoot ratio by expanding root surface area and increasing root tip number.At the hormone level,Dongnong 59 upregulated GA3 content and maintained the balance of key hormone ratios to promote root expansion and antioxidant capacity,while Changji Huangdou had weaker regulation.Metabolomics analysis identified 3 131 differential metabolites,including 2 187 core metabolites between cultivars.Lipids maintained membrane stability,flavonoids alleviated oxidative damage,and amino acids supported root growth,with key pathways such as aminoacyl-tRNA biosynthesis significantly enriched.Correlation analysis confirmed the relationship between amino acid metabolites and root phenotypes.In conclusion,low-nitrogen tolerant soybeans adapt to low nitrogen stress through coordinated regulation of root morphology,hormone homeostasis,and metabolism.This study provides a theoretical basis for the breeding of low-nitrogen tolerant soybeans.关键词
大豆/低氮胁迫/根系构型/内源激素/代谢组学/差异代谢物Key words
soybean/low nitrogen stress/root system architecture/endogenous hormones/metabolomics/differential metabolites引用本文复制引用
管海吉,李志刚,何德鑫,刘鹏,王晓东,刘英,于华荣,张絮颖..低氮胁迫下大豆根系的激素响应及代谢组学分析[J].黑龙江农业科学,2026,(4):16-24,9.基金项目
农业重大科技项目 （）
内蒙古自治区科技计划项目(2023DXZD0002). （2023DXZD0002）
