黑龙江畜牧兽医Issue(5)：60-65,6.DOI:10.13881/j.cnki.hljxmsy.2025.09.0044
大别山牛背最长肌脂肪酸差异相关的基因可变剪接分析
Analysis of alternative splicing of the genes related to fatty acid differences in longest dorsal muscle of Dabieshan cattles
摘要
Abstract
In order to identify and analyze the alternative splicing(AS)events involved in the differential regulation of fatty acids in the longest dorsal muscle of Dabieshan cattle,high-throughput transcriptome sequencing was used to analyze AS events in longissimus dorsi samples with high fatty acid(HF)and low fatty acid(LF),and differentially spliced genes(DSGs)were screened.GO functional annotation and KEGG signaling pathway enrichment analysis were performed on DSGs,and DSGs involved in key KEGG signaling pathways were screened for protein-protein interaction(PPI)network analysis.The results showed that a total of 26,499 AS events were identified,of which 77.43%were exon skipping events.A total of 682 differential AS events were screened,involving 546 differential splicing genes(DSGs).The DSGs mainly participated in metabolic processes,muscle development,and fatty acid metabolism,and were enriched in key signaling pathways such as AMPK and PPAR.The key genes UBC,SMAD2,RHOA,PI4K2B,and TRAF6 played important roles in the regulation of fatty acid metabolism.The results indicated that differential alternative splicing might be involved in regulating the deposition of fatty acids in the longest dorsal muscle of Dabieshan cattle through the interaction of multiple genes.关键词
大别山牛/背最长肌/脂肪酸/转录组/可变剪接Key words
Dabieshan cattle/longest dorsal muscle/fatty acid/transcriptome/alternative splicing分类
农业科技引用本文复制引用
章会斌,金海,赵拴平,杜欣怡,李倩,李庆岗,朱勇,赵克勤,徐磊..大别山牛背最长肌脂肪酸差异相关的基因可变剪接分析[J].黑龙江畜牧兽医,2026,(5):60-65,6.基金项目
国家肉牛牦牛产业技术体系合肥综合试验站项目(CARS-37) （CARS-37）
安徽省科技特派员赋能"秸秆变肉"暨肉牛振兴项目(202513b10050018) （202513b10050018）
安徽省科技攻坚计划项目子课题(202423m10050001) （202423m10050001）
安徽省农业科学院新进博士项目(XJBS-202432) （XJBS-202432）
