湖南生态科学学报2026，Vol.13Issue(1)：10-20,11.DOI:10.3969/j.issn.2095-7300.2026.01.002
四环素与高氯酸盐联合暴露对铜绿微囊藻生理生化活性的影响
Effects of Combined Exposure to Tetracycline and Perchlorate on the Physiological and Biochemical Activities of Microcystis aeruginosa
摘要
Abstract
[Background]The widespread presence and accumulation of perchlorate and tetracycline in water bodies pose a potential threat to aquatic ecosystems.However,the combined toxicity of these two substances on Microcystis aeruginosa remains unclear.[Objective]To investigate the effects of combined exposure to tetracycline and perchlorate on the physiological metabolism of Microcystis aeruginosa,and to provide theoretical support for the risk assessment of their combined exposure in aquatic ecosystems.[Methods]A 96-hour acute exposure experiment was conducted using Microcystis aeruginosa as the test organism.The responses of Microcystis aeruginosa to single and combined exposure to tetracycline and perchlorate were explored by measuring indicators such as photosynthetic pigments,phycobiliproteins,and extracellular polymeric substances(EPS).[Results]Under single exposure to tetracycline and per-chlorate,the growth inhibition rates of algae were 54.74%～66.54%and 29.77%～62.93%,respec-tively;under combined exposure,the inhibition rate was 40.34%～70.48%.The ZIP synergy score of-7.523 indicates that the combined toxicity of tetracycline and perchlorate on algae exhibits an antagonis-tic effect.In addition,under single and combined exposure to tetracycline and perchlorate,the EPS in algae was primarily protein.Under single exposure to tetracycline and perchlorate,the fluorescence in-tensity of extracellular polymer components in algae decreased to a certain extent,while under fluores-cence intensity of the protein component of extracellular polymers showed a increasing trend,and the pro-tein content of extracellular polymers also showed an increasing trend.[Conclusion]Single and combined exposure to tetracycline and perchlorate can inhibit the growth and photosynthesis of Microcystis aeruginosa and change the composition of extracellular polymeric substances.Both substances have strong biological toxicity to Microcystis aeruginosa and exhibit an antagonistic effect.关键词
四环素/高氯酸盐/铜绿微囊藻/联合毒性/拮抗作用/胞外聚合物Key words
tetracycline/perchlorate/Microcystis aeruginosa/combined toxicity/antagonistic effect/extracellular polymeric substances分类
资源环境引用本文复制引用
罗翀,杨思培,秦普丰,吴志斌,梁运姗..四环素与高氯酸盐联合暴露对铜绿微囊藻生理生化活性的影响[J].湖南生态科学学报,2026,13(1):10-20,11.基金项目
湖南农业大学"1515"人才项目 （）
