河南农业科学2026，Vol.55Issue(4)：100-109,10.DOI:10.15933/j.cnki.1004-3268.2026.04.010
不同处理对紫叶小檗种子萌发及其生理特性的影响
Effects of Different Treatments on Seed Germination and Physiological Characteristics of Berberis thunbergii'Atropurpurea'
摘要
Abstract
The Berberis thunbergii'Atropurpurea'seeds exhibit physiological barrier due to their hard seed coat and prolonged dormancy period,which limits the popularization and application.To clarify the effects of different treatments on seed germination characteristics of this species and screen out the optimal treatment to promote seed germination,this study set up various durations(10,15,20,25,30 min,denoted as A1—A5)of sulfuric acid(H2SO4)etching,various durations(15,30,45,60,75 min,denoted as B1—B5)of sodium hydroxide(NaOH)alkali etching and various concentrations(100,150,200,250,300 mg/L,denoted as C1—C5)of gibberellin(GA3)soaking treatments.Seed coat microstructure was observed by scanning electron microscopy,while germination parameters,seedling growth indices,physiological indicators,and endogenous hormone levels were measured.The results showed that H2SO4 etching and GA3 soaking significantly enhanced seed germination and seedling growth,whereas NaOH alkali etching significantly inhibited them.H2SO4 etching improved water and air permeability of seed coat,and significantly increased soluble protein and abscisic acid(ABA)content in the germ.GA3 soaking caused almost no mechanical damage to the seed coat,but significantly increased the soluble protein,soluble sugar and GA3 content in germ by regulating internal physiological metabolism.Although NaOH alkali etching caused no severe seed coat damage,it significantly reduced α-amylase activity and indole-3-acetic acid(IAA)content,while markedly increased ABA levels in germ.The principal component analysis indicated that the comprehensive order of seed germination effect from high to low was C4,C5,C3,C2,C1,A5,A4,A3,A2,A1,CK,B1,B2,B3,B4,B5.Overall,both GA3 soaking and H2SO4 etching treatments could effectively promote the seed germination of Berberis thunbergii'Atropurpurea',and soaking in 250 mg/L GA3 solution for 24 hours exhibited the optimal promoting effect.关键词
紫叶小檗/种子萌发/生理特性/内源激素/种皮微观结构/赤霉素浸种/硫酸酸蚀Key words
Berberis thunbergii'Atropurpurea'/Seed germination/Physiological characteristics/Endogenous hormones/Seed coat microstructure/Gibberellin soaking/Sulfuric acid etching分类
农业科技引用本文复制引用
杨佳佳,刘志敏,周明涛,潘炳锟,刘健..不同处理对紫叶小檗种子萌发及其生理特性的影响[J].河南农业科学,2026,55(4):100-109,10.基金项目
国家重点研发计划项目(2024YFC3012702) （2024YFC3012702）
