核农学报2026，Vol.40Issue(6)：1105-1115,11.DOI:10.11869/j.issn.1000-8551.2026.06.1105
甘薯AUX/LAX基因家族鉴定和非生物胁迫下的表达分析
Identification and Expression Analysis of AUX/LAX Gene Family in Sweetpotato under Abiotic Stresses
摘要
Abstract
The precise regulation of auxin distribution by auxin transporters AUX/LAX(AUXIN RESISTANT1/LIKE AUX1)plays a crucial role in mediating plant growth,development,and responses to abiotic stresses.To characterize the AUX/LAX gene family members in sweetpotato(Ipomoea batatas)and explore their functional roles in stress adaptation,a genome-wide identification and systematic analysis of this gene family were performed in this study.A total of seven AUX/LAX members were identified,distributed across chromosomes 4,5,6,7,9 and 14.All encoded proteins contain the Aa_trans domain,share similar gene structures and conserved motifs,and are predicted to be membrane-localized with 4～11 transmembrane domains.Promoter analysis revealed that these AUX/LAX genes contain 33 types of cis-acting element associated with abiotic and biotic stress responses,hormone responses,and light responsiveness.Expression profiling in vegetable sweetpotato showed that IbAUX/LAX genes were significantly upregulated by eogenous auxin and exhibited distinct expression patterns under salt,drought,and heat stress.Among them,IbAUX2 and IbAUX3 were consistently downregulated under multiple abiotic stresses,whereas IbAUX5,IbAUX6,and IbAUX7 were significantly upregulated at 6 h,1 h and 1 h under drought and heat stress,respectively.Notably,IbAUX1 showed the most pronounced expression changes across all treatments.These findings suggest that AUX/LAX genes may play critical roles in the abiotic stress responses of vegetable sweetpotato.This study provides valuable insights into the functions of the AUX/LAX genes in mediating stress tolerance in vegetable sweetpotato,offering candidate gene resources for the genetic improvement of stress-resistant vegetable sweetpotato varieties.关键词
甘薯/AUX/LAX基因家族/生物信息学/非生物胁迫/基因表达Key words
sweetpotato/AUX/LAX gene family/bioinformatics/abiotic stress/gene expression引用本文复制引用
许正艳,张聪,蒲志刚,冯俊彦,屈会娟,郎涛,任敏,高鹏..甘薯AUX/LAX基因家族鉴定和非生物胁迫下的表达分析[J].核农学报,2026,40(6):1105-1115,11.基金项目
四川省财政自主创新专项(2022ZZCX029),四川省农业科学院科研条件平台建设专项"2025优质高效薯豆关键技术研究与应用"(2025TJPT),四川省农业科学院科研条件平台建设专项"2025生物技术育种工程中心运转费"(51000023Y000008258891),四川省农业科学院科技成果中试熟化与示范转化项目(2025ZSSFJD06) （2022ZZCX029）
