化学试剂2026，Vol.48Issue(5)：32-38,7.DOI:10.13822/j.cnki.hxsj.2026.0074
基于ST-6-2核酸适配体的显色探针用于快速检测新冠病毒S蛋白
Colorimetric Probe Based on ST-6-2 Aptamer for Rapid Detection of SARS-CoV-2 Spike Protein
摘要
Abstract
A colorimetric biosensor based on ST-6-2 aptamer and gold nanoparticles(AuNPs)was developed for the rapid detection of the SARS-CoV-2 spike protein.AuNPs with uniform particle size were synthesized using the sodium-citrate reduction method,and the binding conformational change between the aptamer and the spike protein was verified via circular dichroism spectroscopy.Three conjugation strategies for the DNA functionalization of AuNPs,namely the freeze-thaw,salt-aging,and low-pH methods,were compared.The conjugation efficiency was evaluated based on UV-Vis absorption spectra,and the low-pH method was identified as the optimal strategy.A colorimetric probe(AuNPs@SH-DNA)was constructed by covalently coupling thiol-modified cDNA and Poly T to the surface of AuNPs.Based on the principle of competitive lateral-flow chromatography,a test strip was assembled for the visual detection of the spike protein.Experimental results showed that the sensor exhibited favorable signal response toward the spike protein,thus enabling rapid,low-cost,and point-of-care detection.This colorimetric aptasensor platform provides a novel solution for the point-of-care testing of SARS-CoV-2 and demonstrates significant potential for epidemic screening and decentralized diagnostics.关键词
金纳米颗粒/核酸适配体/新冠病毒S蛋白/比色传感器/侧向流层析Key words
gold nanoparticles/aptamer/SARS-CoV-2 spike protein/colorimetric sensor/lateral flow chromatography分类
化学化工引用本文复制引用
王新妍,朱鹤,嘎鲁,爱军..基于ST-6-2核酸适配体的显色探针用于快速检测新冠病毒S蛋白[J].化学试剂,2026,48(5):32-38,7.基金项目
国家自然科学基金区域联合基金项目(U25A200831) （U25A200831）
国家自然科学基金项目(82160652). （82160652）
