基础医学与临床2026，Vol.46Issue(5)：614-620,7.DOI:10.16352/j.issn.1001-6325.2026.05.0614
睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析
Proteomic and metabolomic analysis of urine in sleep-deprived mouse models
摘要
Abstract
Objective To explore the patho-physiological changes of sleep deprivation(SD)by analyzing the uri-nary proteome and metabolome of sleep-deprived mouse models.Methodse Twelve male mice were randomly divid-ed into a control group and a model group with six in each.Urine samples were collected daily from metabolic cages for 8 consecutive days and subjected to liquid chromatography-mass spectrometry(LC-MS)-based proteomic and metabolomic analyses.Results A total of 855 differential proteins were identified in the urine of sleep-deprived mouse models.Function annotation analysis showed an enrichment in the complement and coagulation cascades and glutathione metabolism pathways.Additionally,158 differential metabolites were identified,which were primarily enriched in metabolic pathways such as pigment metabolic processes,signal transduction/regulation and immune response activation.Integrated proteomic and metabolomic analysis revealed that the core function pathways were glutathione metabolism and complement and coagulation cascades.Conclusions Changes in SD-related protein and metabolite metabolic pathways provide novel insights into the mechanisms underlying sleep deprivation.关键词
睡眠剥夺/蛋白质组学/代谢组学/尿液Key words
sleep deprivation/proteomics/metabolomics/urine分类
医药卫生引用本文复制引用
张玉雪,刘婷婷,何梦真,胡斯奇,李军,孙伟..睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析[J].基础医学与临床,2026,46(5):614-620,7.基金项目
国家自然科学基金(82170524,31901039) （82170524,31901039）
