基础医学与临床2026，Vol.46Issue(5)：637-642,6.DOI:10.16352/j.issn.1001-6325.2026.05.0637
IDH1作为非经典RNA结合蛋白维持急性髓系白血病细胞系HL-60的分化阻滞
IDH1 functions as a noncanonical RNA-binding protein to maintain differentiation blockade in acute myeloid leukemia cell line HL-60
摘要
Abstract
Objective To investigate the novel mechanism by which isocitrate dehydrogenase 1(IDH1)suppresses differentiation in acute myeloid leukemia(AML)HL-60 cells.Methods Potential RNA-binding proteins were ini-tially screened through literature review and database analyses,leading to the selection of IDH1 as the candidate of interest.RNA immunoprecipitation followed by sequencing(RIP-seq)was performed to identify RNAs bound by IDH1 in HL-60 cells.In parallel,IDH1 was knocked down via lentiviral transduction,and transcriptome profiling was conducted by RNA sequencing(RNA-seq)to assess global gene expression changes induced by IDH1 depletion.Integrated analysis of RIP-seq and RNA-seq data was further carried out to systematically characterize the expression patterns of IDH1-bound transcripts following IDH1 knockdown.Results IDH1 was found to bind a broad spectrum of RNA molecules in HL-60 cells,with mRNAs representing the predominant class.Knockdown of IDH1 resulted in marked alterations in the cellular gene expression profile.Integrated analysis revealed that IDH1-bound transcripts exhibited an overall downregulation upon IDH1 depletion and were significantly enriched in genes associated with differentiation blockade in leukemic cells.Conclusions IDH1 functions as a non-canonical RNA-binding protein that participates in the regulation of differentiation-associated genes in AML,thereby contributing to the maintenance of the undifferentiated state of leukemia cells.关键词
异柠檬酸脱氢酶 1(IDH1)/急性髓系白血病/分化阻滞/RNA 免疫沉淀测序Key words
isocitrate dehydrogenase 1(IDH1)/acute myeloid leukemia/differentiation blockade/RNA immunoprecipitation se-quencing分类
医药卫生引用本文复制引用
邹宾宾,陈仲扬,马艳妮,杨嘉宾,余佳..IDH1作为非经典RNA结合蛋白维持急性髓系白血病细胞系HL-60的分化阻滞[J].基础医学与临床,2026,46(5):637-642,6.基金项目
国家自然科学基金(92268205) （92268205）
