基础医学与临床2026，Vol.46Issue(5)：643-650,8.DOI:10.16352/j.issn.1001-6325.2026.05.0643
CHO细胞降温培养过程中的动态转录物组特征
Dynamic transcriptomic characteristics of CHO cells during temperature downshift culture
摘要
Abstract
Objective To investigate the dynamic transcriptomic changes of Chinese hamster ovary(CHO)cells during reduced-temperature culture and to characterize the temporal patterns and potential regulatory features of gene expression under low-temperature conditions.Methods CHO cells were cultured under standard conditions(37℃)and reduced temperature(30℃)separately.Cells were harvested at 0 h,24 h,and 48 h for RNA se-quencing(RNA-seq).Both preprocessing and downstream analyses of the sequencing data were performed,inclu-ding differential gene expression analysis,temporal expression clustering,functional enrichment.Protein-protein in-teraction(PPI)network analysis was performed to evaluate alterations in key signaling pathways during temperature reduction.Results Temperature reduction markedly altered the transcriptomic profile of CHO cells.Temporal clustering identified six distinct gene expression patterns,mainly associated with protein translation and ribosome biogenesis,cell cycle regulation,and vesicle transport.Biomolecular interaction network analysis revealed that translation-related molecules and membrane structure/signaling-related nodes formed relatively concentrated interac-tion modules,exhibiting differential temporal dynamics.Conclusions CHO cells undergo pronounced time-depend-ent transcriptomic remodeling during reduced-temperature culture characterized by stage-specific adjustments in translation-related and membrane structure-related processes.These findings provide transcriptome-level evidence for cellular state transitions and production-related regulation in low-temperature.关键词
中国仓鼠卵巢(CHO)细胞/降温培养/转录物组学/时间序列分析/生物分子互作网络Key words
Chinese hamster ovary(CHO)cell/temperature downshift/transcriptomics/time-series analysis/biomolecular interac-tion network analysis分类
生物科学引用本文复制引用
保永莉,夏婉娉,王晓悦,吴汝成,李书翔,李雪媛,陈阳..CHO细胞降温培养过程中的动态转录物组特征[J].基础医学与临床,2026,46(5):643-650,8.基金项目
国家自然科学基金(32570755) （32570755）
