基础医学与临床2026，Vol.46Issue(5)：651-657,7.DOI:10.16352/j.issn.1001-6325.2026.05.0651
长链非编码RNA SNHG1与剪接因子相互作用影响红细胞分化
Long non-coding RNA SNHG1 interacting with splicing factors influences erythroid differentiation
摘要
Abstract
Objective To investigate the function and molecular mechanism of long non-coding RNA(lncRNA)small nucleolar RNA host gene 1(SNHG1)in erythroid differentiation.Methods During erythroid differentiation of CD34+hematopoietic stem/progenitor cells derived from human umbilical cord blood,expression of SNHG1 was suppressed.Erythroid differentiation was assessed by flow cytometry with surface markers CD71 and CD235a,while the mRNA expression of globin genes was measured by reverse transcription quantitative PCR(RT-qPCR).Nucle-ar-cytoplasm fractionation and RNA fluorescence in situ hybridization(RNA-FISH)were applied to determine the sub-cellular localization of SNHG1.RNA pull-down and RNA immunoprecipitation(RIP)assays were applied to identify SNHG1-interacting proteins.Results Suppression of SNHG1 significantly decreased the proportion of CD71+/CD235a+erythroid progenitor cells(P＜0.001)and reduced the expression of globingene(P＜0.01).SNHG1 was found to be predominantly localized in the nucleus and interacted with multiple splicing regulators,including SFPQ and NONO.Conclusions SNHG1 may affect erythroid differentiation by recruiting splicing-related proteins such as SFPQ and NONO and thereby regulating RNA splicing and processing.关键词
长链非编码 RNA(lncRNA)/红系分化/RNA 剪接Key words
long non-coding RNA(lncRNA)/erythroid differentiation/RNA splicing分类
医药卫生引用本文复制引用
沈欣妍,郭嘉,马艳妮,余佳,刘思琪..长链非编码RNA SNHG1与剪接因子相互作用影响红细胞分化[J].基础医学与临床,2026,46(5):651-657,7.基金项目
国家自然科学基金(32470847) （32470847）
