基础医学与临床2026，Vol.46Issue(6)：772-776,5.DOI:10.16352/j.issn.1001-6325.2026.06.0772
NAT10对32D细胞周期和凋亡的影响
Effects of NAT10 on the cell cycle and apoptosis of 32D cells
摘要
Abstract
Objective To investigate the function of NAT10 in the mouse myeloid progenitor cell line 32D.Methods The NAT10 gene was knocked out in 32D cells using CRISPR/Cas9 technology,and the knockout efficiency was verified by RT-qPCR and Western blot.Flow cytometry was employed to assess cell cycle distribution and apopto-sis,and the experimental results were statistically analyzed.Results A NAT10 knockout 32D cell model was successfully established.Compared with the control group,NAT10 knockout resulted in a marked reduction in the percentage of cells in the G1 phase accompanied by a significant increase in the percentage of cells in the S phase.Meanwhile,the proportion of early and late apoptotic cells was significantly reduced(P＜0.05).Conclusions NAT10 plays an important role in the proliferation and survival of 32D cells.Its loss can lead to cell cycle activation and inhibition of apoptosis,suggesting that NAT10 may be involved in the regulation of myeloid progenitor cell growth and in limiting their excessive proliferation.关键词
NAT10/髓系祖细胞/细胞周期/细胞凋亡Key words
NAT10/myeloid progenitor cells/cell cycle/apoptosis分类
生物科学引用本文复制引用
顾娆,李卫倩,王芳,余佳..NAT10对32D细胞周期和凋亡的影响[J].基础医学与临床,2026,46(6):772-776,5.基金项目
国家自然科学基金(92268205) （92268205）
