基础医学与临床2026，Vol.46Issue(6)：807-814,8.DOI:10.16352/j.issn.1001-6325.2026.06.0807
Mcart1基因敲除对小鼠巨噬细胞代谢及表型的影响
Effects of Mcart1 gene knockout on metabolism and phenotype of mouse macrophages
摘要
Abstract
Objective To investigate the effects of Mcart1 gene knockout on macrophage metabolism and phenotype.Methods Two types of bone marrow-derived macrophages(BMDMs)were obtained from Mcart1flox/flox and Mcart1Lyz2-cre mice.Basal respiration of both cell types was measured using a Seahorse analyzer.The activity of α-ketoglutarate dehydrogenase(OGDH)was assessed in Mcart1 stably knockout RAW264.7 cells(Mcart1-/--RAW264.7)and wild-type RAW264.7 cells(RAW264.7).The two types of BMDMs were induced into M1 mac-rophages using LPS,and whole-cell reactive oxygen species(ROS)levels were measured by flow cytometry,while dynamic expression of inflammatory mediators was monitored by qPCR.The two types of BMDMs were also induced into M2 macrophages using IL-4,and expression of M2 anti-inflammatory and tissue repair mediators was measured by qPCR,while protein levels of classical M2 activation markers were detected using Western blot.Results Com-pared with Mcart1flox/flox BMDMs,Mcart1 knockout significantly reduced basal respiration in BMDMs,and Mcart1 stable knockout decreased OGDH activity in macrophages(P＜0.01).Mcart1 knockout significantly enhanced ROS production in M1 macrophages and upregulated early inflammatory mediators Il1b,Tnf,and Nos2(P＜0.05),while having no significant effect on the expression of M2 anti-inflammatory and tissue repair mediators Arg1,Mrc1,Ret-nla,Cd163,Fn1,or classical activation proteins p-Stat6 and p-Stat3 in M2 macrophages.Conclusions Mcart1 knockout significantly suppresses basal respiration in macrophages,inhibits OGDH enzyme activity,increases oxi-dative stress and inflammatory mediator levels in M1 macrophages,and does not significantly affect functional medi-ator levels in M2 macrophages.关键词
Mcart1/NAD+/活性氧/α-酮戊二酸脱氢酶/巨噬细胞极化Key words
Mcart1/NAD+/reactive oxygen species/α-ketoglutarate dehydrogenase/macrophage polarization分类
医药卫生引用本文复制引用
王莹莹,张楷晗,王钰铖,鞠瑞,郭磊..Mcart1基因敲除对小鼠巨噬细胞代谢及表型的影响[J].基础医学与临床,2026,46(6):807-814,8.基金项目
呼吸和共病全国重点实验室开放课题基金(2060204) （2060204）
中国医学科学院医药与健康科技创新工程探索项目(2025-I2M-TS-05) （2025-I2M-TS-05）
