实用临床医药杂志2026，Vol.30Issue(7)：60-66,7.DOI:10.7619/jcmp.20256766
七氟烷调控S100A8/HPX/Fra-1通路抑制脊髓缺血再灌注损伤中的铁死亡
Sevoflurane inhibits ferroptosis in spinal cord ischemia-reperfusion injury by regulating S100A8/HPX/Fra-1 pathway
摘要
Abstract
Objective To investigate whether sevoflurane affects the mechanisms related to fer-roptosis in spinal cord ischemia-reperfusion injury(SCIR)by influencing the functions of S100 calci-um-bindingprotein A8(S100A8),hemopexin(HPX)genes,and Fos-related antigen-1(Fra-1).Methods A total of 45 6-week-old male SD rats were randomly divided into sham operation group,model group,and sevoflurane group,with 15 rats in each group.The SCIR model was successfully replicated in the model group and the sevoflurane group.The sevoflurane group inhaled 2.4％sevoflurane 3 h before model replication,while the model group inhaled an equal volume of air.The Basso-Beattie-Bresnahan(BBB)locomotor rating scale score was evaluated 96 h after model replica-tion.HE staining was used to observe morphological changes in spinal cord tissue.TUNEL staining was employed to count the apoptotic rate.The expression levels of S100A8 mRNA,HPX mRNA,and Fra-1 mRNA were detected by qRT-PCR.The expression levels of ferroptosis markers glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11)were detected by Western blot.The levels of reactive oxygen species and malondialdehyde were measured using a kit method.Results Com-pared with the sham operation group,the model group showed significantly decreased BBB scores,expression levels of GPX4 and SLC7A11,and significantly increased apoptotic rate,expression lev-els of S100A8 mRNA,HPX mRNA,and Fra-1 mRNA,as well as reactive oxygen species and ma-londialdehyde levels(P＜0.05).Compared with the model group,the sevoflurane group exhibited significantly increased BBB scores,expression levels of GPX4 and SLC7A11,and significantly de-creased apoptotic rate,expression levels of S100A8 mRNA,HPX mRNA,and Fra-1 mRNA,as well as reactive oxygen species and malondialdehyde levels(P＜0.05).Conclusion Sevoflurane inhibits the process of cellular ferroptosis in SCIR through the S100A8/HPX/Fra-1 axis.关键词
七氟烷/脊髓缺血再灌注损伤/铁死亡/S100钙结合蛋白A8/血红素结合蛋白/Fos相关抗原-1/谷胱甘肽过氧化物酶4/溶质载体家族7成员11Key words
sevoflurane/spinal cord ischemia-reperfusion injury/ferroptosis/S100 calcium-binding protein A8/hemopexin/Fos-related antigen-1/glutathione peroxidase 4/solute carrier fam-ily 7 member 11分类
医药卫生引用本文复制引用
玉素普·努尔麦麦提,麦斯坦古力·曼苏拉,程虎,亚力·亚森,李爱梅,黄一丹,吴建江..七氟烷调控S100A8/HPX/Fra-1通路抑制脊髓缺血再灌注损伤中的铁死亡[J].实用临床医药杂志,2026,30(7):60-66,7.基金项目
新疆维吾尔自治区科学技术厅"天山创新团队计划"(2025D14008) （2025D14008）
