江苏大学学报（自然科学版）2026，Vol.47Issue(3)：292-299,8.DOI:10.3969/j.issn.1671-7775.2026.03.006
基于细胞电化学生物传感器的辣椒素检测方法
Detection of capsaicin based on electrochemical cellular bio-sensors
摘要
Abstract
As the main pungent ingredient in chili products,the content of capsaicin is one of the important markers for evaluating the quality of chili peppers.Developing straightforward,rapid and sensitive method to determine capsaicin levels that aligns with human sensory capabilities is highly significant.The A549 cells were selected as sensitive element,and the electrochemical bio-sensors with cell/PPy/PEDOT:PSS/AuNPs modified ITO was successfully prepared.To improve the sensitivity of sensor,the electrochemical deposition of AuNPs was performed for 120 s with the concentration of PEDOT:PSS in the PPy/PEDOT:PSS conductive gel of 3.0％and the density of A549 cells of 5×105 cells/mL,and the electrochemical cellular sensors constructed under these conditions showed the best sensing performance.The results show that the detection of capsaicin is realized by differential pulse voltammetry,and the limit of detection is 2.53 μmol/L.The linear range of the assay is 30 to 210 μmol/L.The relative standard deviation of the five times of the assay is 3.25％,and the stability of the assay on the 10th day is 91.19％.The bio-sensor provides new method for the detection of capsaicin.关键词
辣椒素/细胞/生物传感器/导电聚合物水凝胶/检测Key words
capsaicin/cell/bio-sensor/conductive polymer hydrogel/detection分类
信息技术与安全科学引用本文复制引用
邹小波,高立莹,李志华,黄晓玮..基于细胞电化学生物传感器的辣椒素检测方法[J].江苏大学学报（自然科学版）,2026,47(3):292-299,8.基金项目
国家自然科学基金资助项目(32372465,32272407) （32372465,32272407）
江苏省自然科学基金优秀青年基金资助项目(BK20220111) （BK20220111）
