军事医学2026，Vol.50Issue(4)：260-267,8.DOI:10.7644/j.issn.1674-9960.2025-00263
射频辐射对小鼠学习记忆信息处理和海马CA1锥体神经元钙活动模式的影响
Effects of radiofrequency radiation on learning and memory information processing and calcium activity patterns of hippocampal CA1 pyramidal neurons in mice
摘要
Abstract
Objective To investigate the real-time biological effects of 2650 MHz radiofrequency radiation(RFR)on learning and memory as well as calcium activity patterns of hippocampal CA1 pyramidal neurons of mice.Methods Adult male C57BL/6N mice were randomly assigned to control(CON)and RFR groups before being exposed to 2650 MHz RFR for 3 hours in an electromagnetic reverberation chamber.Within 1 hour after exposure,changes in learning and memory were assessed using novel object recognition(NOR),object location recognition(OLR),and temporal order recognition(TOR)tests.Calcium activity of CA1 pyramidal neurons was monitored before,during,and after exposure using genetically encoded calcium imaging combined with fiber photometry.Neuronal activation was evaluated by c-Fos immunofluorescence staining.Results Compared with the CON group,RFR-exposed mice showed significantly reduced preference indices for target objects in the NOR(P<0.001),OLR(P<0.01),and TOR(P<0.01)tests within 1 hour post-exposure.During RFR exposure,neuronal calcium signals exhibited an abnormal high-frequency but low-amplitude pattern,characterized by increased event frequency(P<0.05)and decreased single-event amplitude(P<0.05).Furthermore,the immunofluorescence intensity of c-Fos in the hippocampal CA1 region was significantly reduced(P<0.05).Conclusion 2650 MHz radiofrequency radiation exposure can induce immediate effects,such as abnormalities in calcium signaling patterns and suppressed excitability of pyramidal neurons in the hippocampal CA1 region,ultimately leading to impaired learning and memory functions.关键词
射频辐射/实时生物效应/小鼠/学习记忆/海马/锥体神经元/钙活动Key words
radiofrequency radiation/real-time biological effects/mice/learning and memory/hippocampus/pyramidal neurons/calcium activity分类
医药卫生引用本文复制引用
方豪阳,冯婷,李刻勤,杨雪枫,赵梦瑶,郝延辉,李杨..射频辐射对小鼠学习记忆信息处理和海马CA1锥体神经元钙活动模式的影响[J].军事医学,2026,50(4):260-267,8.基金项目
北京市自然科学基金青年项目(7244457) （7244457）
