军事医学2026，Vol.50Issue(4)：268-276,9.DOI:10.7644/j.issn.1674-9960.2026-00008
颈部淋巴结无针注射对不同制剂的入脑递送效率比较研究
Comparison of brain delivery efficiency of diverse formulations administered via cervical lymph node needle-free injection
摘要
Abstract
Objective To investigate the efficacy of needle-free injection(NFIT)through cervical lymph nodes as a novel strategy for drug delivery into the brain,and compare its efficiency for different drugs.Methods Using Evans blue as an injectant in vitro,the differences in drug diffusion between NFIT and needle-dependent injection were studied.By using sodium fluorescein as an injectant in vivo,the tissue distribution of sodium fluorescein in mice over time was evaluated after injection by different means before the dynamic changes of drug accumulation in the brain and lymphatic tissues were observed.Three representative drugs-cyanine5.5(Cy5.5,a small molecule compound),Cy5.5-bovine serum albumin(Cy5.5-BSA,a protein drug),and Cy5.5-hyaluronic acid(Cy5.5-HA,a high molecular weight polymer)were used to evaluate the brain delivery efficiency of different drugs via NFIT.Results In vitro diffusion experiments found a more rapid and uniform diffusion of drugs after NFIT while in vivo experiments showed that the delivery efficiency of fluorescein sodium through intracervical lymphatic NFIT was significantly higher in the cortex and hippocampus of mice than with traditional injection methods.A comparison of three representative drugs confirmed that intracervical lymphatic NFIT was an effective way to deliver small molecule compounds and protein drugs to the brain,but was not workable for polymers of high molecular mass.Conclusion Intracervical lymphatic NFIT can efficiently mediate the brain delivery of small molecules and protein drugs,which promises to be a drug delivery method for the application of such drugs to the brain.关键词
无针注射/颈部淋巴结/淋巴系统/血脑屏障Key words
needle-free injection/cervical lymph nodes/lymphatic system/blood brain barrier分类
医药卫生引用本文复制引用
董晓倩,宋梦文,张睿瑶,张妮,王玉记,刘志强,彭滢,乔鑫..颈部淋巴结无针注射对不同制剂的入脑递送效率比较研究[J].军事医学,2026,50(4):268-276,9.基金项目
国家自然科学基金联合基金项目(U25A2068) （U25A2068）
国家自然科学基金面上项目(82371394) （82371394）
北京自然科学基金项目(7232348) （7232348）
