产自四川的环纹硫黄菌的生物学特性
Biological characteristics of Laetiporus zonatus from Sichuan Province,Southwest China
摘要
Abstract
A pure culture strain(SSJ01)was obtained by isolating and purifying an orange edible mushroom purchased from a market in Kangding City,Garzê Tibetan Autonomous Prefecture,Sichuan Province,and was identified as Laetiporus zonatus based on morphological characteristics and ITS sequence analysis.The mycelial growth conditions of this fungus were optimized through single-factor and orthogonal experiments,and a preliminary domestication study was conducted.The results of the single-factor experiments indicated that the optimal carbon source for mycelial growth was sucrose,the optimal nitrogen source was yeast extract,the optimal C/N was 30:1,the optimal temperature was 25℃,the optimal pH was 5.0,and the optimal light conditions were complete darkness;no inorganic salts were required.The orthogonal experiment showed that the effects of the tested factors on mycelial growth rate were ranked as temperature>pH>carbon source>nitrogen source.The optimal combination of culture condition was selecting fructose as the carbon source and peptone as the nitrogen source,and cultivation under 25℃and pH 5.0.For domestication cultivation,substrate composed of 94%cottonseed hulls,5%wheat bran,and 1%lime with moisture content of 55%-60%was adopted.After 30-35 day incubation in the dark at 25℃,the plastic cultivation bags fully colonised by mycelium were transferred to the conditions of 20-25℃,85%-90%relative humidity,illumination of about 12 hours under diffused light and ventilation of three times per day.In about 20-30 days clustered early fruiting bodies formed.This research provides basic data for further studies of domestication,cultivation,development and utilization of Laetiporus zonatus.关键词
硫黄菌/分离鉴定/培养条件优化/人工栽培Key words
Laetiporus/isolation and identification/optimization of culture condition/artificial cultivation引用本文复制引用
林芳萍,尤霞,陈羽璐,郭芷恩,黄芷悦,周雨菲,生书晶,王超群..产自四川的环纹硫黄菌的生物学特性[J].菌物学报,2026,45(5):116-130,15.基金项目
广东省教育厅2025年广东省重点建设学科科研能力提升项目(2025ZDJS134) （2025ZDJS134）
广州市科技计划(2025A04J4220) （2025A04J4220）
广东第二师范学院数字化课程资源建设提升计划(食用菌栽培学) （食用菌栽培学）
广东第二师范学院大学生创新创业训练计划(2026103) （2026103）
广东第二师范学院生态学科研团队项目(广东二师[2020]58 号)This work was supported by the 2025 Key Construction Discipline Research Capacity Enhancement Project of the Department of Education of Guangdong Province(2025ZDJS134),the Guangzhou Science and Technology Project(2025A04J4220),the Digital Course Resources Construction and Enhancement Project of Guangdong University of Education(Edible Mushroom Cultivation),the College Students'Innovative Entrepreneurial Training Plan Program of Guangdong University of Education(2026103),and the Project of Ecology Research Team at Guangdong University of Education(Guangdong University of Education[2020]No.58). （广东二师[2020]58 号）
