解放军医学院学报2026，Vol.47Issue(2)：124-132,9.DOI:10.12435/j.issn.2095-5227.25110302
包封负载EGCG的介孔聚多巴胺纳米粒子的壳聚糖/F127复合水凝胶的构建、表征及体外促成骨分化与安全性评价
Construction,characterization,in vitro osteogenic differentiation and biosafety evaluation of chitosan/F127 composite hydrogels encapsulating mesoporous polydopamine nanoparticles loaded with EGCG
摘要
Abstract
Background Bone defect repair is a common challenge in clinical orthopedics,and traditional repair materials are limited by drawbacks such as low biological activity and poor drug controlled-release performance.Objective To construct a composite hydrogel capable of promoting bone defect repair by combining the properties of epigallocatechin gallate(EGCG)and mesoporous polydopamine(MPDA),while leveraging the excellent biocompatibility and mechanical properties of chitosan/F127 hydrogel.Methods Cells treated with regular culture medium were used as the control(Ctrl)group.EGCG-loaded MPDA(MPDA@EGCG)nanoparticles were prepared,and their particle size,Zeta potential,and morphology were characterized using scanning electron microscopy(SEM)and a nanoparticle size and Zeta potential analyzer.Subsequently,the aforementioned nanoparticles were incorporated into chitosan/F127 hydrogel to fabricate the composite hydrogel(MPDA@EGCG-CF).The morphology,rheological properties,in vitro release behavior,biosafety,and in vitro osteogenic capacity of the composite hydrogel were evaluated via SEM,a rheometer,the CCK-8 assay,live/dead cell staining,hemolysis test,alkaline phosphatase staining with quantitative analysis,alizarin red staining with quantitative analysis,and Western blot analysis.Results The MPDA@EGCG-CF hydrogel was successfully constructed.In vitro release studies demonstrated that the composite hydrogel could achieve sustained local release of EGCG for more than one week.Morphological observations via SEM revealed that the hydrogel exhibited favorable morphological characteristics with a porous structure.Cell experiments and hemolysis test confirmed the good biosafety of the composite material.Results from alkaline phosphatase(ALP)staining,alizarin red staining,and Western blot analysis indicated that,compared with the Ctrl group and the hydrogel group encapsulating non-drug-loaded MPDA,the MPDA@EGCG-CF hydrogel displayed significantly enhanced in vitro osteogenic differentiation capacity(P＜0.05).Conclusion The constructed osteoconductive chitosan/F127 composite hydrogel encapsulating EGCG-loaded MPDA can realize long-term local release of EGCG and remarkably promote osteogenic differentiation in vitro,exhibiting promising application potential for bone regeneration.关键词
骨再生/表没食子儿茶素没食子酸酯/MC3T3-E1细胞/壳聚糖/介孔聚多巴胺Key words
bone regeneration/epigallocatechin gallate/MC3T3-E1 cells/chitosan/mesoporous polydopamine分类
医药卫生引用本文复制引用
刘鑫,刘光波,李冬冬,孙小涵,游家良,白云,赵珮汝,彭江..包封负载EGCG的介孔聚多巴胺纳米粒子的壳聚糖/F127复合水凝胶的构建、表征及体外促成骨分化与安全性评价[J].解放军医学院学报,2026,47(2):124-132,9.基金项目
国家重点研发计划(2024YFA1108605) （2024YFA1108605）
