精准医学杂志2026，Vol.41Issue(2)：100-105,6.DOI:10.13362/j.jpmed.202641030
circCDYL对大鼠心脏成纤维细胞活化的影响及其作用机制研究
Effect of circCDYL on the activation of rat cardiac fibroblasts and its underlying mechanism
摘要
Abstract
Objective To investigate the effect of circCDYL on the activation of rat cardiac fibroblasts and its underlying mechanism.Methods Cardiac fibroblasts were isolated from neonatal SD rats and cultured.The cells were divided into groups A to D.Group A was not treated with reagents,Group B was treated with angiotensin Ⅱ(Ang Ⅱ),Group C was transfected with si-NC and then treated with Ang Ⅱ,Group D was transfected with si-circCDYL and then treated with Ang Ⅱ.RT-qPCR was used to detect the relative expression level of circCDYL and the relative mRNA expression levels of extracellular matrix-related genes(Col1a1,Col3a1,FN1).Cell proliferation was assessed using EdU staining and the CCK-8 assay.The expression level of α-SMA was detected by immunofluorescence staining.The phosphorylation level of Smad2 protein was detected by Western blot.Results RT-qPCR showed that the relative expression of circCDYL in rat cardiac fibroblasts gradually increased over time after Ang Ⅱ treatment for 6,12,24 and 48 h(F=338.30,P＜0.05).Compared with group C,group D showed significantly reduced relative expression of circCDYL(F=16.06,q=5.98,P＜0.05).EdU staining showed that the proportion of EdU-positive cells in group D was significantly reduced compared with that in group C(F=14.76,q=7.19,P＜0.05).CCK-8 assay showed that between 12 and 72 h of culture,the absorbance values of group D were significantly lower than those of group C(F=24.19-303.60,q=25.35-31.75,P＜0.05).RT-qPCR,immunofluorescence staining,and Western blot respectively showed that compared with group C,group D exhibited significantly reduced relative mRNA expression levels of Col1a1,Col3a1,and FN1,mean fluorescence intensity of α-SMA,and p-Smad2/Smad2 ratio(F=16.66-86.28,q=5.48-10.99,P＜0.05).Conclusion circCDYL promotes the acti-vation of rat cardiac fibroblasts,and the mechanism may be related to the promotion of Smad2 phosphorylation in the TGF-β/Smad signaling pathway.关键词
肌成纤维细胞/肌细胞,心脏/RNA,环状/circCDYL/Smad蛋白质类/基因表达调控/信号传导Key words
Myofibroblasts/Myocytes,cardiac/RNA,circular/circCDYL/Smad proteins/Gene expression regula-tion/Signal transduction分类
医药卫生引用本文复制引用
李嘉欣,李萌阳..circCDYL对大鼠心脏成纤维细胞活化的影响及其作用机制研究[J].精准医学杂志,2026,41(2):100-105,6.基金项目
山东省自然科学基金项目(ZR2024MH255) （ZR2024MH255）
