精准医学杂志2026，Vol.41Issue(2)：125-128,134,5.DOI:10.13362/j.jpmed.202641032
miR-892b对骨肉瘤细胞增殖、迁移和凋亡的影响及其机制
Effect and mechanism of miR-892b on the proliferation,migration,and apoptosis of osteosarcoma cells
摘要
Abstract
Objective To investigate the effect and mechanism of miR-892b on the proliferation,migration,and apopto-sis of osteosarcoma cells.Methods RT-qPCR was used to measure the relative expression level of miR-892b in osteosarcoma cell lines(MG-63,U2OS)and the human osteoblast cell line hFOB1.19.U2OS cells were divided into group A(transfected with mimic NC),group B(transfected with miR-892b mimic),group C(transfected with inhibitor NC),and group D(transfected with miR-892b inhibitor).CCK-8 assay was used to measure the absorbance value of each group at 24,48,and 72 hours of culture after transfection;Transwell assay was used to measure the number of migrating cells in each group;Western blot was used to measure the expression levels of the apoptosis-related protein Bax and the key protein p-NF-κB in the NF-κB signaling pathway.Results RT-qPCR showed that compared with the hFOB1.19 cells,the MG-63 and U2OS cells showed a significant reduction in the relative expression level of miR-892b(F=73.59,tDunnett=7.83,9.92,P＜0.05).CCK-8 assay showed that at 24,48,and 72 hours of cul-ture,group B had a significantly lower absorbance value than group A,while group D had a significantly higher absorbance value than group C(F=5.91-31.23,P＜0.05).Compared with group A,group B had a significant reduction in the number of migrating cells,a significant increase in the protein expression level of Bax,and a significant reduction in the protein expression level of p-NF-κB;compared with group C,group D had a significant increase in the number of migrating cells,a significant reduction in the pro-tein expression level of Bax,and a significant increase in the protein expression level of p-NF-κB(t=3.64-7.16,P＜0.05).Con-clusion There is a relatively low expression level of miR-892b in osteosarcoma cells,and miR-892b may regulate the proliferation,migration,and apoptosis of osteosarcoma cells by affecting the NF-κB pathway.关键词
骨肉瘤/微RNAs/miR-892b/细胞增殖/细胞运动/细胞凋亡/NF-κB/信号传导Key words
Osteosarcoma/MicroRNAs/miR-892b/Cell proliferation/Cell movement/Apoptosis/NF-kappa B/Signal transduction分类
医药卫生引用本文复制引用
薛生山,陈多平,王建东,魏建仝..miR-892b对骨肉瘤细胞增殖、迁移和凋亡的影响及其机制[J].精准医学杂志,2026,41(2):125-128,134,5.基金项目
甘肃省联合科研基金一般项目(24JRRG019) （24JRRG019）
甘肃省教育厅产业支撑计划项目(2025CYZC-061) （2025CYZC-061）
张掖市科技计划项目(ZY2024JS29) （ZY2024JS29）
河西学院校长基金创新团队项目(CXTD002) （CXTD002）
