昆虫学报2026，Vol.69Issue(4)：495-508,14.DOI:10.16380/j.kcxb.2026.04.003
水稻黑条矮缩病毒与介体昆虫和寄主水稻互作机制及抗病育种研究进展
Research progress on the interaction mechanisms of rice black-streaked dwarf virus with its vector insect and host rice,and disease resistance breeding
摘要
Abstract
Rice blac k-streaked dwarf virus disease(RBSDVD),caused by rice black-streaked dwarf virus(RBSDV),is a devastating viral disease that poses a significant threat to rice production in China and East Asia,historically causing severe yield losses.RBSDV belongs to the genus Fijivirus in the family Spinareoviridae and is primarily transmitted in nature by the small brown planthopper(Laodelphax striatellus)in a persistent and propagative manner.In recent years,significant progress has been made in understanding the mechanisms of virus-vector-host rice interactions and in rice breeding for disease resistance.Regarding virus-vector interactions,the RBSDV outer capsid protein P10 induces autophagy by promoting glyceraldehyde-3-phosphate dehydrogenase(GAPDH)phosphorylation in vector and subsequently hijacks phosphatidylinositol 3,5-bisphosphate[PI(3,5)P2]to escape autophagic degradation.The viral matrix protein P9-1 facilitates viral accumulation by competitively binding to the 26S proteasome subunit RPN8 of vector,thereby inhibiting the ubiquitin-proteasome pathway.Additionally,the virus exploits the L.striatellus sugar transporter LsST6 to cross the midgut barrier,and modulates vector miRNAs and lncRNAs to facilitate infection.In terms of virus-host rice interactions,the virus-encoded protein P6 functions as an RNA silencing suppressor,impairing host antiviral defenses by mediating the degradation of the rice SUMO-conjugating enzyme OsSCE1b.Furthermore,RBSDV fine-tunes plant hormone signaling networks via proteins such as P5-1,P7-2 and P8.These mechanisms include suppressing jasmonic acid biosynthesis,blocking gibberellin signal transduction by binding to GID2 and interfering with the auxin pathway,collectively promoting viral infection and causing plant dwarfing.Meanwhile,rice endogenous pathways involving nitric oxide,lncRNAs and epigenetic modifications have also been reported to participate in the regulation of RBSDV infections.In this article,in the field of resistance breeding,we summarized the progress in discovering resistant germplasms,such as W44 and Tetep,and highlighted the cloning and functional characterization of key resistance genes like OsAP47 and OsGLK1.We also discussed the application outcomes of modern biotechnologies,including marker-assisted selection(MAS),gene editing and RNA interference(RNAi)in breeding for resistance.Future research should systematically elucidate the tripartite virus-vector-plant interactions and co-evolutionary mechanisms,accelerate the mining and utilization of resistance genes,address the impact of climate change and rhizosphere microecology on disease epidemics,and establish a long-term dynamic monitoring and early warning system for diseases.This review aims to provide theoretical references and technical support for the integrated management of RBSDVD and the breeding of resistant rice varieties.关键词
水稻黑条矮缩病毒/水稻/灰飞虱/互作机制/抗性育种/抗性基因Key words
Rice black-streaked dwarf virus/rice/Laodelphax striatellus/interaction mechanism/resistance breeding/resistance gene分类
生物科学引用本文复制引用
李晨羊,杨文轩,朱凤,周彤..水稻黑条矮缩病毒与介体昆虫和寄主水稻互作机制及抗病育种研究进展[J].昆虫学报,2026,69(4):495-508,14.基金项目
国家重点研发计划项目(2023YFD1400300) （2023YFD1400300）
广西科技基地和人才专项(桂科AC22035090) （桂科AC22035090）
国家自然科学基金项目(32361143519,32372513) （32361143519,32372513）
