临床与实验病理学杂志2026，Vol.42Issue(4)：454-462,9.DOI:10.13315/j.cnki.cjcep.2026.04.006
基于pc-tNGS技术检测支气管肺泡灌洗液中病原微生物协助病理诊断的价值
Value of probe capture-based targeted next-generation sequencing for detecting pathogenic microorganisms in bronchoalveolar lavage fluid
摘要
Abstract
Objective To evaluate the diagnostic value of probe capture-based targeted next-generation sequenc-ing(pc-tNGS)for detecting pathogenic microorganisms in bronchoalveolar lavage fluid(BALF)from patients with pul-monary infections.Methods BALF samples were collected from 39 patients with pulmonary infections.The micro-bial load and pathogen spectrum were analyzed using pc-tNGS,and the results were compared with those obtained by conventional microbiological testing(CMT)and multiplex PCR-based targeted next-generation sequencing(mp-tNGS).Results The pathogen detection rate of pc-tNGS was significantly higher than that of CMT(97.44%vs 74.36%,P<0.01).In particular,pc-tNGS markedly improved the detection rates of fungi and bacteria compared with CMT(76.92%vs 7.69%,P<0.01).The overall pathogen detection rate of pc-tNGS was comparable to that of mp-tNGS(97.44%vs 97.30%,P>0.05).However,differences in sensitivity for specific pathogens were observed:mp-tNGS showed higher sensitivity for Mycoplasma pneumoniae and Candida species,whereas pc-tNGS demonstrated superior performance in detecting Mycobacterium species.In addition,pc-tNGS simultaneously identified antimicro-bial resistance genes and virulence genes in BALF samples,providing useful information for clinical antimicrobial therapy and subsequent management.Conclusion pc-tNGS is an effective method for pathogen detection in BALF samples from patients with pulmonary infections.Compared with conventional microbiological methods,it provides higher detection efficiency and broader coverage of Mycobacterium species than mp-tNGS.This technique can provide more accurate,comprehensive,and cost-effective etiological diagnostic information,thereby improving the diagnostic efficiency of pulmonary infectious diseases.关键词
肺部疾病/感染/探针捕获靶向下一代测序/肺泡灌洗液/病原微生物Key words
pulmonary disease/infectious/probe capture-based targeted next-generation sequencing/bronchoalveo-lar lavage fluid/pathogen分类
医药卫生引用本文复制引用
李婕,王茜,易祥华,刘文慧,宋曙,雷正瑶,王顺利,张素霞,孟晓伟..基于pc-tNGS技术检测支气管肺泡灌洗液中病原微生物协助病理诊断的价值[J].临床与实验病理学杂志,2026,42(4):454-462,9.基金项目
同济大学附属同济医院第六周期重点学科基金(ZDTS24-BL) The Sixth Cycle of Key Discipline Fund of Tongji Hospital Affiliated to Tongji University(ZDTS24-BL) （ZDTS24-BL）
