岭南现代临床外科2026，Vol.26Issue(2)：75-83,9.DOI:10.3969/j.issn.1009-976X.2026.02.001
COL7A1基因通过激活PI3K/AKT通路促进胆道肿瘤的发生发展
COL7A1 promotes the development of biliary tract cancer by activating the PI3k/AKT pathway
摘要
Abstract
Objective To investigate the expression characteristics,prognostic value,biological func-tions,and regulatory mechanism of COL7A1 on the PI3K/AKT signaling pathway in biliary tract cancer.Methods Using TCGA cholangiocarcinoma transcriptome data,differential expression analysis,Cox regression,and LASSO regression were performed to screen key prognostic genes.A COL7A1 stable knockdown model was established in HCCC-9810 cells.Cell proliferation,invasion,and migration were assessed by CCK-8,Transwell,and wound healing assays.A subcutaneous xenograft tumor model in nude mice was established to evaluate the effect of COL7A1 knockdown on tumor growth.Immunohistochemis-try was used to detect CD8 ⁺ T cell infiltration.Western blotting was performed to detect the expression levels of AKT and p-AKT in cells to verify the regulatory effect of COL7A1 on the PI3K/AKT pathway.Results COL7A1 expression was significantly upregulated in cholangiocarcinoma tissues,and high expression was associated with poor patient prognosis.Knockdown of COL7A1 significantly inhibited the proliferation,invasion,and migration of HCCC-9810 cells,as well as the growth of subcutaneous xenograft tumors in nude mice.Following COL7A1 knockdown,CD8⁺ T cell infiltration in xenograft tissues was increased.At the cellular level,p-AKT phosphorylation levels were reduced after COL7A1 knock-down,while total AKT expression remained unchanged.Conclusion COL7A1 is highly expressed in biliary tract cancer and promotes tumor cell proliferation,invasion,migration,and in vivo tumorigenesis by positively regulating the phosphorylation of the PI3K/AKT pathway.Its expression level is negatively correlated with CD8⁺T cell infiltration,suggesting that COL7A1 may serve as a prognostic biomarker and therapeutic target for biliary tract cancer.关键词
胆道肿瘤/COL7A1/PI3K/AKT信号通路/增殖/侵袭/预后Key words
biliary tract cancer/COL7A1/PI3K/AKT signaling pathway/proliferation/invasion/prognosis分类
医药卫生引用本文复制引用
刘叶,梁浇,余昊,周志恒,徐鋆耀..COL7A1基因通过激活PI3K/AKT通路促进胆道肿瘤的发生发展[J].岭南现代临床外科,2026,26(2):75-83,9.基金项目
吴阶平教育基金会(320.6750.2025-06-235) （320.6750.2025-06-235）
