辽宁中医药大学学报2026，Vol.28Issue(6)：13-18,6.DOI:10.13194/j.issn.1673-842X.2026.06.003
桂枝茯苓汤调控METTL3/NGF通路改善硼替佐米致周围神经病变机制研究
Mechanism Study on the Regulation of METTL3/NGF Pathway by Guizhi Fuling Decoction(桂枝茯苓汤)in Improving Bortezomib-Induced Peripheral Neuropathy
摘要
Abstract
Objective This study aims to investigate the impact of Guizhi Fuling Decoction(桂枝茯苓汤)on bortezomib-induced peripheral neuropathy through regulating the METTL3/NGF signaling pathway,and to clarify its potential intervention role in promoting nerve injury repair.Methods Eight-week-old female C57BL/6 mice were randomly divided into control group,model group(bortezomib group)and treatment group(bortezomib+Guizhi Fuling Decoction).The model group and the treatment group were intraperitoneally injected with bortezomib.The three groups were tested for behavioral tests(thermal withdrawal latency+cold hyperalgesia).Neurophysiological examination and sciatic nerve motor nerve conduction velocity;the morphology of sciatic nerve was observed by HE staining and electron microscopy.The protein expression levels of METTL3 and NGF in sciatic nerve were detected by Western blot method.The localization and distribution of METTL3 and NGF in tissues were observed by immunohistochemistry method.The serum NGF level was detected by ELISA method.Results Compared with the control group,the latency of thermal hyperalgesia in the model group was significantly shortened(P<0.01),the number of cold stimulation foot lifts was significantly increased(P<0.01).And the nerve conduction velocity of sciatic nerve was significantly decreased(P<0.01).The arrangement of sciatic nerve fibers was disordered and the myelin sheath structure was damaged.The density of nerve fibers,the proportion of myelin sheath formation and the ratio of axon/myelin sheath thickness(g ratio)were significantly decreased(P<0.05).The expression of METTL3 protein in sciatic nerve tissue was significantly increased(P<0.01),the expression of NGF protein was significantly decreased(P<0.01),and the serum NGF level was significantly decreased(P<0.01).Compared with the model group,the thermal withdrawal latency of the treatment group was prolonged(P<0.01),the number of cold stimulation foot lifts was reduced(P<0.01).And the nerve conduction velocity of sciatic nerve was restored(P<0.01).The structural integrity of sciatic nerve myelin sheath was improved,and the morphological indexes were increased(P<0.05).METTL3 protein expression decreased(P<0.01),NGF protein expression and serum NGF level increased(P<0.01).Conclusion Guizhi Fuling Decoction can alleviate bortezomib-induced peripheral neuropathy in C57BL/6 mice,and its mechanism may be associated with the regulation of the METTL3/NGF signaling pathway.关键词
周围神经病变/桂枝茯苓汤/METTL3/NGFKey words
peripheral neuropathy/Guizhi Fuling Decoction(桂枝茯苓汤)/METTL3/NGF分类
医药卫生引用本文复制引用
周汝黛,崔兴..桂枝茯苓汤调控METTL3/NGF通路改善硼替佐米致周围神经病变机制研究[J].辽宁中医药大学学报,2026,28(6):13-18,6.基金项目
国家自然科学基金面上项目(82274491) （82274491）
山东省自然科学基金项目(ZR2024MH009) （ZR2024MH009）
山东省自然科学基金创新发展联合基金项目(ZR2023LZL009) （ZR2023LZL009）
济南市临床医学科技创新计划项目(202225014) （202225014）
