麦类作物学报2026，Vol.46Issue(5)：595-603,9.DOI:10.7606/j.issn.1009-1041.2026.05.04
小麦耐低温相关NAC转录因子分析与表达
Analysis and Expression of NAC Transcription Factors Related to Low Temperature Tolerance in Wheat
摘要
Abstract
To investigate the functional characteristics of low-temperature tolerance-related NAC tran-scription factors in wheat,a low-temperature-tolerant variety Mazhamai and a low-temperature-sensi-tive variety Jimai 22 were used for transcriptome sequencing,and 31 candidate NAC transcription fac-tors potentially associated with low-temperature tolerance were screened.Bioinformatics analysis showed that 12 of the TaNAC proteins are basic proteins(pI＞7),while the others are acidic proteins(pI＜7).All proteins are hydrophilic and mainly localized in the nucleus.The secondary structure was dominated by random coils.Collinearity analysis revealed that there were extensive collinear rela-tionships between NAC transcription factors in wheat and rice.Promoter analysis indicated that the promoter regions of these NAC transcription factor genes contained various cis-acting elements involved in stress responses and hormone signaling,among which TaNAC048-A1 harbored the most abundant regulatory elements.Under low-temperature stress,the expression levels of TaNAC048-D1,TaN-AC048-A1,TaNAC015-B4,TaNAC039-D1,TaNAC104-D2,TaNAC048-B1,TaNAC001-B1,and TaN-AC001-A1 were significantly up-regulated in Mazhamai.In Jimai 22,TaNAC048-D1,TaNAC039-D1,and TaNAC104-D2 were significantly up-regulated,while TaNAC048-B1 was significantly down-regu-lated,and the remaining four genes showed no significant changes.The transcriptome sequencing da-ta were verified by qRTPCR.These TaNAC transcription factor genes may play important roles in the response of wheat to low-temperature stress.关键词
小麦/低温胁迫/NAC转录因子/表达分析Key words
Wheat/Low-temperature stress/NAC transcription factor/Expression analysis分类
农业科技引用本文复制引用
马艳,姚立蓉,李葆春,王化俊,杜佛力,汪军成,张宏,司二静,杨轲,孟亚雄,马小乐..小麦耐低温相关NAC转录因子分析与表达[J].麦类作物学报,2026,46(5):595-603,9.基金项目
甘肃省科技厅重点研发项目(25YFNA032) （25YFNA032）
甘肃省创新训练项目(S202510733020) （S202510733020）
甘肃省科技计划联合计划基金项目(24JRRA840) （24JRRA840）
2025大学生创新创业训练项目(2025) （2025）
现代寒旱特色农业种业攻关项目(ZYGG-2025-3,ZYGG-2025-12-3) （ZYGG-2025-3,ZYGG-2025-12-3）
甘肃省重点人才项目(2023RCXM70) （2023RCXM70）
中央引导地方科技发展资金项目(25ZYJA002) （25ZYJA002）
甘肃省科技计划项目(24CXNA038) （24CXNA038）
甘肃省自然科学基金重点项目(24JRRA637) （24JRRA637）
甘肃省教育厅产业支撑计划项目(2021CYZC-12) （2021CYZC-12）
甘肃农业大学伏羲青年英才计划项目(Gaufx-03Y06,GAUfx-04Y011) （Gaufx-03Y06,GAUfx-04Y011）
国重实验室开放基金项目(GSCS-2021-05) （GSCS-2021-05）
