生态与农村环境学报2026，Vol.42Issue(5)：662-670,9.DOI:10.19741/j.issn.1673-4831.2025.0333
长期增温对土壤酶活性的影响
The Influence of Long-term Warming on Soil Enzyme Activities
摘要
Abstract
Global warming is a key environmental factor affecting the soil carbon and nitrogen cycle process,and its influ-ence effect varies depending on different soil types.However,the regulatory mechanism of extracellular enzymes in the soil within it remains unclear.To study the response of extracellular enzyme activities in soil to long-term warming,continuous warming simulation experiments were carried out in this study.Six common soil,namely red soil,laterite,black soil,brown soil,purple soil and loess,were selected as the research objects.The Open warming system(FATI)was used to conduct all-day warming of winter wheat throughout its growth period.The activities of soil sucrase(S-SC),cellobiose hydrolase(CBH),β-glucosidase(βG),β-xylitase(βX),urease(S-UE),β-acetylglucosidase(NAG),phosphatase(AP)and catalase(CAT)were determined.The results show that the warming(+2℃)treatment significantly enhanced the soil enzyme activities,and there were extremely significant differences in the response characteristics of different enzymes in different soil types(P＜0.01).The sucrase activity in black soil showed the strongest temperature sensitivity,with an increase of 104.7%in its activity,significantly higher than that of other soil types(P＜0.01).The urease activity in loess responded most significantly to temperature increase,with an increase of 79.22%in its activity,significantly higher than that of the normal temperature control treatment(P＜0.01).There were significant differences in carbon cycle enzymes,nitrogen cycle enzymes and phosphorus cycle enzymes among different soil types(P＜0.01).Warming increased the activ-ities of carbon cycle enzymes and nitrogen cycle enzymes in red soil,while reduced the activities of nitrogen cycle enzymes and phosphorus cycle enzymes in purple soil.Among the carbon cycle enzymes,the βX enzyme and catalase in alkaline soil were significantly higher than those in other soil types(P＜0.01).Among nitrogen cycle enzymes,the urease activity in acidic soil was significantly higher than that in other soil types(P＜0.01),and among phosphorus cycle enzymes,the phosphatase activity in neutral soil was significantly higher than that in other soil types(P＜0.01).Whether or not the temperature increases,the microorganisms in the six types of soil are jointly limited by carbon(C)and phosphorus(P).Enzyme stoichiometric analysis reveals that the enzyme activity vector angles of all soil samples were significantly greater than 45°(P＜0.05),which indicates that microorganisms were more sensitive to the limitation of P element.These results indicate that there is an interaction between warming and soil type on the activities of soil carbon,nitrogen and phosphorus cycling enzymes,and particular attention to phosphorus cycling enzymes is needed.关键词
增温/土壤/酶活性/酶化学计量比/冬小麦Key words
warming/soil/enzyme activity/enzyme stoichiometry ratio/winter wheat分类
农业科技引用本文复制引用
兰嘉蕊,苗淑杰,乔云发,李天,李虎山,吴钥..长期增温对土壤酶活性的影响[J].生态与农村环境学报,2026,42(5):662-670,9.基金项目
江苏省碳达峰碳中和科技创新专项资金项目(BE2023400-02) （BE2023400-02）
江苏省农业农村领域重大关键技术攻关项目(BE2022425) （BE2022425）
