农业生物技术学报2026，Vol.34Issue(6)：1141-1152,12.DOI:10.3969/j.issn.1674-7968.2026.06.001
青稞HvvCYP90A基因的克隆及其抗盐功能初步分析
Cloning and Preliminary Analysis of Salt Resistance Function of HvvCYP90A Gene in Hordeum vulgare var.nudum
摘要
Abstract
The function of the CYP90A subfamily genes in monocots remains unclear,but they are likely involved in the biosynthesis of the plant hormone brassinosteroids(BRs).This study used Hordeum vulgare var.nudum,a characteristic crop of the plateau,as the experimental material.By utilizing its genomic database,the CYP90A subfamily gene of H.vulgare var.nudum was identified,its cDNA sequence was cloned,and bioinformatics and expression pattern analyses were conducted.Additionally,genetic transformation of Arabidopsis thaliana was performed to explore its functions of salt resistance.The results showed that only one CYP90A subfamily gene was found in H.vulgare var.nudum genome,named HvvCYP90A.The coding region of the HvvCYP90A gene was 1 542 bp long,encoding 513 amino acids.The coding region similarity between the CYP90A genes of H.vulgare var.nudum and H.vulgare was as high as 96.76%,but there are significant differences in the promoter sequences,with HvvCYP90A having unique response elements for drought and circadian rhythm.Evolutionary analysis revealed that the CYP90A gene was differentiated between monocots and dicots,but there was no obvious regulation in the number of genes among different species.As the seedlings of H.vulgare var.nudum grew,the expression of HvvCYP90A gradually decreased in the roots but increased in the leaves.Moreover,the expression of HvvCYP90A was inhibited by exogenous 28-homobrassinolide(HBR).Additionally,after transferring HvvCYP90A into A.thaliana,the root length of transgenic A.thaliana was significantly increased compared with the wild type(P＜0.05)under normal and salt stress conditions.It was speculated that the HvvCYP90A gene regulated the salt tolerance of A.thaliana by participating in the biosynthesis of BRs.This study provides reference for the mechanism of brassinosteroid regulated salt stress adaptation of H.vulgare var.nudum.关键词
青稞/油菜素内酯/CYP90A/生物合成/抗盐性Key words
Hordeum vulgare var.nudum/Brassinosteroids/CYP90A/Biosynthesis/Salt tolerance分类
农业科技引用本文复制引用
冯洋,赵艺云,段瑞君,袁怡玲,任慧珍,司建萍..青稞HvvCYP90A基因的克隆及其抗盐功能初步分析[J].农业生物技术学报,2026,34(6):1141-1152,12.基金项目
青海省科技厅应用基础研究项目(2022-ZJ-948Q) （2022-ZJ-948Q）
