农业生物技术学报2026，Vol.34Issue(6)：1153-1163,11.DOI:10.3969/j.issn.1674-7968.2026.06.002
黄秋葵NCED基因的鉴定及其在果荚中的表达分析
Identification of NCED Gene in Hibiscus esculentus and Its Expression Analysis in Pods
摘要
Abstract
Abscisic acid(ABA)serves as a pivotal hormone in plants,regulating the entire process of plant growth,development,and stress responses.Nine-cis-epoxycarotenoid dioxygenase(NCED)acts as the rate-limiting enzyme in ABA biosynthesis,directly determining its biosynthetic efficiency and content.The identification and analysis of the NCED gene in Hibiscus esculentus are crucial for elucidating the mechanism of ABA-mediated regulation of pod senescence.In this study,the HeNCED gene in H.esculentus was identified and analyzed using bioinformatics.The results revealed that the full length of HeNCED gene(GenBank No.PZ350883)was 2 196 bp,with an open reading frame(ORF)of 1 803 bp encoding 600 amino acids.The protein was localized in the cytoplasm,exhibiting acidic,hydrophilic,and unstable properties.Sequence similarity analysis showed over 88%identity with homologous proteins from Gossypium anomalum,G.raimondii,G.arboreum,and H.syriacus,indicating high conservation.qPCR analysis of pod samples at different developmental stages and post-harvest treated with exogenous ABA and nordihydroguaiaretic acid(NDGA)demonstrated a significant positive correlation between HeNCED expression and ABA content(P＜0.01).It was hypothesized that HeNCED regulated secondary wall thickening in H.esculentus pods via modulating ABA synthesis.This study preliminarily revealed the molecular mechanism of HeNCED in Hibiscus esculentus pod aging,which can provide a theoretical basis and genetic resources for molecular breeding,cultivation,and post-harvest storage of H esculentus.关键词
黄秋葵/9-顺式-环氧类胡萝卜素双氧合酶(NCED)/脱落酸(ABA)/纤维素/木质素Key words
Hibiscus esculentus/9-cis-epoxy carotenoid dioxygenase(NCED)/Abscisic acid(ABA)/Cellulose/Lignin分类
农业科技引用本文复制引用
李永平,郑多乐,张荟,裘波音,薛珠政,朱海生,温庆放..黄秋葵NCED基因的鉴定及其在果荚中的表达分析[J].农业生物技术学报,2026,34(6):1153-1163,11.基金项目
福建省属公益类科研院所基本科研专项(2023R1029002) （2023R1029002）
国家大宗蔬菜产业技术体系福州综合试验站项目(CARS-23-G51) （CARS-23-G51）
福建省农业高质量发展超越"5511"协同创新工程(XTCXGC2021003) （XTCXGC2021003）
