农业生物技术学报2026，Vol.34Issue(6)：1164-1172,9.DOI:10.3969/j.issn.1674-7968.2026.06.003
黄秋葵肉桂酸-4-羟化酶基因AeC4H的克隆与功能分析
Cloning and Functional Analysis of Cinnamate 4-hydroxylase Gene AeC4H in Okra(Abelmoschus esculentus)
摘要
Abstract
Cinnamate 4-hydroxylase(C4H)plays a central role in the phenylpropanoid pathway,catalyzing the conversion of cinnamate to 4-coumarate,which serves as the essential starting substrate for the biosynthesis of secondary metabolites such as lignin and flavonoids.However,the precise functional mechanisms of C4H in okra(Abelmoschus esculentus)remain incompletely characterized.This study isolated and characterized the C4H gene(named AeC4H)(NCBI accession No.SAMN44959506)from the okra cultivar'Lyuwuxing',conducting comprehensive analyses of its molecular properties,expression profiles,subcellular localization,and biological functions.Bioinformatic analysis revealed that AeC4H possessed a 1 506 bp ORF encoding 501 amino acids,exhibiting the highest sequence identity(97.23%)with its ortholog from Hibiscus cannabinus(Malvaceae).The encoded protein contained conserved P450 family domains,including the characteristic heme-binding motif and substrate recognition sites.AeC4H exhibited preferential expression in young roots,stems,and developing seeds compared with other tissues,as quantified by qRT-PCR.Subcellular localization studies confirmed the endoplasmic reticulum localization of AeC4H,aligning with the known compartmentalization of phenylpropanoid metabolism.Overexpression of AeC4H in Arabidopsis thaliana extremely significantly increased total flavonoids content in transgenic plants(P＜0.01).This study provides the systematic characterization of AeC4H in okra,offering novel insights into flavonoid metabolism and valuable genetic resources for molecular breeding programs targeting nutritional quality improvement in this important vegetable crop.关键词
黄秋葵/肉桂酸-4-羟化酶(C4H)/基因克隆/表达分析/异源过表达Key words
Abelmoschus esculentus/Cinnamate 4-hydroxylase(C4H)/Gene cloning/Expression analysis/Heterologous overexpression分类
农业科技引用本文复制引用
张艳,孙婷,刘玉珊,秦恒山,何小岗,杨马进,张景荣..黄秋葵肉桂酸-4-羟化酶基因AeC4H的克隆与功能分析[J].农业生物技术学报,2026,34(6):1164-1172,9.基金项目
四川省农业科学院"5+1"农业前沿技术攻关项目(5+1QYGG005) （5+1QYGG005）
四川省省级公益性科研单位基本科研业务费专项资金(24JBKY07) （24JBKY07）
