农业与技术2026，Vol.46Issue(5)：14-20,7.DOI:10.19754/j.nyyjs.20260530003
响应面优化酶辅助超声提取红豆越橘花色苷工艺
Response Surface Optimization of Enzyme-Assisted Ultrasonic Extraction of Anthocyanins from Vaccinium vitis-idaea L.
摘要
Abstract
Taking the fruits of Vaccinium vitis-idaea L.as the raw material and the anthocyanins extraction yield as the evaluation index,single-factor experiments were conducted to evaluate the effects of enzyme type,ultrasonic time,ultrasonic temperature,ethanol concentration,enzyme dosage,enzymatic hydrolysis temperature,enzymatic hydrolysis time,and solid-to-liquid ratio on the anthocyanins extraction yield.Response surface methodology(RSM)was used to optimize the extraction process.The results showed that the anthocyanin extraction yield of the cellulase-treated group was 11.5%and 7.6%higher than that of the non-enzyme control group and pectinase group,respectively.The basic process conditions optimized by single-factor experiments were as follows:cellulase dosage of 1%,enzymatic hydrolysis temperature of 35℃,enzymatic hydrolysis time of120min,and solid-to-liquid ratio of 1∶20 g·mL-1.The optimal process optimized by RSM was determined as follows:ultrasonic time of 21.69 min,ultrasonic temperature of 39.67℃,and ethanol concentration of 51.95%Under these conditions,the predicted anthocyanin extraction yield was 3.54 mg·g-1,and the actual verified extraction yield was 3.53±0.02 mg·g-1,which was in good agreement with the predicted value.The cellulase-assisted ultrasonic extraction process established in this study provides a theoretical basis and technical support for the efficient extraction of anthocyanins from Vaccinium vitis-idaea L.cellulase-assisted ultrasonic extraction technology for anthocyanins from Vaccinium vitis-idaea L.,which provides a theoretical and technical foundation for the in-depth development and utilization of anthocyanins.关键词
红豆越橘/花色苷/酶辅助超声提取/响应面优化Key words
Vaccinium vitis-idaea L./anthocyanins/enzyme-assisted ultrasound extraction/response surface optimization分类
农业科技引用本文复制引用
梁景涛,荆柏彤,张卓,吕新铭,高雪霞,袁肖寒,顾成波..响应面优化酶辅助超声提取红豆越橘花色苷工艺[J].农业与技术,2026,46(5):14-20,7.基金项目
教育部、国家外国专家局高等学校学科创新引智计划(项目编号:B20088) （项目编号:B20088）
